NDLI: Complex formation by positive and negative translational regulators of GCN4.

Please wait, while we are loading the content...

Year: 2016, Volume: 36

Year: 2015, Volume: 36

Year: 2015, Volume: 35

Year: 2014, Volume: 35

Year: 2014, Volume: 34

Year: 2013, Volume: 33

Year: 2012, Volume: 32

Year: 2011, Volume: 31

Year: 2010, Volume: 30

Year: 2009, Volume: 29

Year: 2008, Volume: 28

Year: 2007, Volume: 27

Year: 2006, Volume: 26

Year: 2005, Volume: 25

Year: 2004, Volume: 24

Year: 2003, Volume: 23

Year: 2002, Volume: 23

Year: 2002, Volume: 22

Year: 2001, Volume: 21

Year: 2000, Volume: 20

Year: 1999, Volume: 19

Year: 1998, Volume: 18

Year: 1997, Volume: 17

Year: 1996, Volume: 16

Year: 1995, Volume: 15

Year: 1994, Volume: 14

Year: 1993, Volume: 13

Year: 1992, Volume: 12

Year: 1991, Volume: 11

Year: 1991, Volume: 11, Issue: 12

Year: 1991, Volume: 11, Issue: 11

Year: 1991, Volume: 11, Issue: 10

Year: 1991, Volume: 11, Issue: 9

Year: 1991, Volume: 11, Issue: 8

Year: 1991, Volume: 11, Issue: 7

Year: 1991, Volume: 11, Issue: 6

Effects of excess centromeres and excess telomeres on chromosome loss rates.

A common transcriptional activator is located in the coding region of two replication-dependent mouse histone genes.

Synergistic transcriptional activation by CTF/NF-I and the estrogen receptor involves stabilized interactions with a limiting target factor.

In vivo stimulation of a chimeric promoter by binding sites for nuclear factor I.

Pheromone response elements are necessary and sufficient for basal and pheromone-induced transcription of the FUS1 gene of Saccharomyces cerevisiae.

Alternative splicing generates isoforms of the met receptor tyrosine kinase which undergo differential processing.

cis-acting sequences required for expression of the divergently transcribed Drosophila melanogaster Sgs-7 and Sgs-8 glue protein genes.

The yeast SLY gene products, suppressors of defects in the essential GTP-binding Ypt1 protein, may act in endoplasmic reticulum-to-Golgi transport.

Organizational analysis of elav gene and functional analysis of ELAV protein of Drosophila melanogaster and Drosophila virilis.

NF-AB, a liver-specific and cytokine-inducible nuclear factor that interacts with the interleukin-1 response element of the rat alpha 1-acid glycoprotein gene.

SPT5, an essential gene important for normal transcription in Saccharomyces cerevisiae, encodes an acidic nuclear protein with a carboxy-terminal repeat.

Molecular cloning and expression of the type 1 and type 2 murine receptors for tumor necrosis factor.

Ribosome association of GCN2 protein kinase, a translational activator of the GCN4 gene of Saccharomyces cerevisiae.

Enhancer-binding activity of the tal-1 oncoprotein in association with the E47/E12 helix-loop-helix proteins.

The Steel/W transduction pathway: kit autophosphorylation and its association with a unique subset of cytoplasmic signaling proteins is induced by the Steel factor.

Organization of the murine and human interleukin-7 receptor genes: two mRNAs generated by differential splicing and presence of a type I-interferon-inducible promoter.

Different classes of polyadenylation sites in the yeast Saccharomyces cerevisiae.

A generic intron increases gene expression in transgenic mice.

Mutations in the yeast RNA14 and RNA15 genes result in an abnormal mRNA decay rate; sequence analysis reveals an RNA-binding domain in the RNA15 protein.

Identification of a GTPase-activating protein homolog in Schizosaccharomyces pombe.

DNA repair in the c-myc proto-oncogene locus: possible involvement in susceptibility or resistance to plasmacytoma induction in BALB/c mice.

Translation initiation factor 5A and its hypusine modification are essential for cell viability in the yeast Saccharomyces cerevisiae.

Degradation of a developmentally regulated mRNA in Xenopus embryos is controlled by the 3' region and requires the translation of another maternal mRNA.

Suppression of tumor formation in vivo by expression of the JE gene in malignant cells.

A ras effector domain mutant which is temperature sensitive for cellular transformation: interactions with GTPase-activating protein and NF-1.

In vitro analysis of the tissue plasminogen activator promoter reveals a GC box-binding activity present in murine brain but undetectable in kidney and liver.

BALB/c-3T3 fibroblasts resistant to growth inhibition by beta interferon exhibit aberrant platelet-derived growth factor, epidermal growth factor, and fibroblast growth factor signal transduction.

Strand breaks without DNA rearrangement in V (D)J recombination.

Multiple dispersed spontaneous mutations: a novel pathway of mutation in a malignant human cell line.

Translational control of discoidin lectin expression in drsA suppressor mutants of Dictyostelium discoideum.

Maturation of polycistronic pre-mRNA in Trypanosoma brucei: analysis of trans splicing and poly(A) addition at nascent RNA transcripts from the hsp70 locus.

The juxtamembrane regions of the epidermal growth factor receptor and gp185erbB-2 determine the specificity of signal transduction.

GCD2, a translational repressor of the GCN4 gene, has a general function in the initiation of protein synthesis in Saccharomyces cerevisiae.

Complex formation by positive and negative translational regulators of GCN4.

The PHO84 gene of Saccharomyces cerevisiae encodes an inorganic phosphate transporter.

Nur77 is differentially modified in PC12 cells upon membrane depolarization and growth factor treatment.

Functional interaction of hybrid response elements with wild-type and mutant steroid hormone receptors.

Partial characterization of a cellular factor that regulates the double-stranded RNA-dependent eIF-2 alpha kinase in 3T3-F442A fibroblasts.

Involvement of single-stranded tails in homologous recombination of DNA injected into Xenopus laevis oocyte nuclei.

Characterization of recombination intermediates from DNA injected into Xenopus laevis oocytes: evidence for a nonconservative mechanism of homologous recombination.

An inducible cytoplasmic factor (AU-B) binds selectively to AUUUA multimers in the 3' untranslated region of lymphokine mRNA.

Structure, chromosome location, and expression of the human smooth muscle (enteric type) gamma-actin gene: evolution of six human actin genes.

The CYC8 and TUP1 proteins involved in glucose repression in Saccharomyces cerevisiae are associated in a protein complex.

The human CCG1 gene, essential for progression of the G1 phase, encodes a 210-kilodalton nuclear DNA-binding protein.

Telomeric location of Giardia rDNA genes.

Transcription of muscle-specific genes is repressed by reactivation of pp60v-src in postmitotic quail myotubes.

Developmental regulation of the alpha-mannosidase gene in Dictyostelium discoideum: control is at the level of transcription and is affected by cell density.

Pulsed-field electrophoresis of megabase-sized DNA.

A 32-kilodalton protein binds to AU-rich domains in the 3' untranslated regions of rapidly degraded mRNAs.

Ca2+ is essential for multistep activation of the heat shock factor in permeabilized cells.

The Saccharomyces cerevisiae SRK1 gene, a suppressor of bcy1 and ins1, may be involved in protein phosphatase function.

Dominant positive and negative selection using a hygromycin phosphotransferase-thymidine kinase fusion gene.

The glucocorticoid receptor binds to a sequence overlapping the TATA box of the human osteocalcin promoter: a potential mechanism for negative regulation.

Cell cycle-specific expression and nuclear binding of DNA polymerase alpha.

Template-directed primer extension catalyzed by the Tetrahymena ribozyme.

Cloning by differential screening of a Xenopus cDNA that encodes a kinesin-related protein.

Year: 1991, Volume: 11, Issue: 5

Year: 1991, Volume: 11, Issue: 4

Year: 1991, Volume: 11, Issue: 3

Year: 1991, Volume: 11, Issue: 2

Year: 1991, Volume: 11, Issue: 1

Year: 1990, Volume: 10

Year: 1989, Volume: 9

Year: 1988, Volume: 8

Year: 1987, Volume: 7

Year: 1986, Volume: 6

Year: 1985, Volume: 5

Year: 1984, Volume: 4

Year: 1983, Volume: 3

Year: 1982, Volume: 2

Year: 1981, Volume: 1

Complex formation by positive and negative translational regulators of GCN4.

Content Provider	PubMed Central
Author	Cigan, A. M. Foiani, M. Hannig, E. M. Hinnebusch, A. G.
Abstract	GCN4 is a transcriptional activator of amino acid biosynthetic genes in Saccharomyces cerevisiae whose expression is regulated by amino-acid availability at the translational level. GCD1 and GCD2 are negative regulators required for the repression of GCN4 translation under nonstarvation conditions that is mediated by upstream open reading frames (uORFs) in the leader of GCN4 mRNA. GCD factors are thought to be antagonized by the positive regulators GCN1, GCN2 and GCN3 in amino acid-starved cells to allow for increased GCN4 protein synthesis. Previous genetic studies suggested that GCD1, GCD2, and GCN3 have closely related functions in the regulation of GCN4 expression that involve translation initiation factor 2 (eIF-2). In agreement with these predictions, we show that GCD1, GCD2, and GCN3 are integral components of a high-molecular-weight complex of approximately 600,000 Da. The three proteins copurified through several biochemical fractionation steps and could be coimmunoprecipitated by using antibodies against GCD1 or GCD2. Interestingly, a portion of the eIF-2 present in cell extracts also cofractionated and coimmunoprecipitated with these regulatory proteins but was dissociated from the GCD1/GCD2/GCN3 complex by 0.5 M KCl. Incubation of a temperature-sensitive gcdl-101 mutant at the restrictive temperature led to a rapid reduction in the average size and quantity of polysomes, plus an accumulation of inactive 80S ribosomal couples; in addition, excess amounts of eIF-2 alpha, GCD1, GCD2, and GCN3 were found comigrating with free 40S ribosomal subunits. These results suggest that GCD1 is required for an essential function involving eIF-2 at a late step in the translation initiation cycle. We propose that lowering the function of this high-molecular-weight complex, or of eIF-2 itself, in amino acid-starved cells leads to reduced ribosomal recognition of the uORFs and increased translation initiation at the GCN4 start codon. Our results provide new insights into how general initiation factors can be regulated to affect gene-specific translational control.
Starting Page	3217
File Format	PDF
ISSN	10985549
e-ISSN	10985549
Journal	Molecular and Cellular Biology
Issue Number	6
Volume Number	11
Language	English
Publisher Date	1991-06-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Biology

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in