NDLI: Promoter elements determining weak expression of the GAL4 regulatory gene of Saccharomyces cerevisiae.

Please wait, while we are loading the content...

Year: 2016, Volume: 36

Year: 2015, Volume: 36

Year: 2015, Volume: 35

Year: 2014, Volume: 35

Year: 2014, Volume: 34

Year: 2013, Volume: 33

Year: 2012, Volume: 32

Year: 2011, Volume: 31

Year: 2010, Volume: 30

Year: 2009, Volume: 29

Year: 2008, Volume: 28

Year: 2007, Volume: 27

Year: 2006, Volume: 26

Year: 2005, Volume: 25

Year: 2004, Volume: 24

Year: 2003, Volume: 23

Year: 2002, Volume: 23

Year: 2002, Volume: 22

Year: 2001, Volume: 21

Year: 2000, Volume: 20

Year: 1999, Volume: 19

Year: 1998, Volume: 18

Year: 1997, Volume: 17

Year: 1996, Volume: 16

Year: 1995, Volume: 15

Year: 1994, Volume: 14

Year: 1993, Volume: 13

Year: 1993, Volume: 13, Issue: 12

Year: 1993, Volume: 13, Issue: 11

Year: 1993, Volume: 13, Issue: 10

Year: 1993, Volume: 13, Issue: 9

Year: 1993, Volume: 13, Issue: 8

CpG island mapping of a mouse double-minute chromosome.

Either alpha-tubulin isogene product is sufficient for microtubule function during all stages of growth and differentiation in Aspergillus nidulans.

Inhibition of thyrotropin-stimulated DNA synthesis by microinjection of inhibitors of cellular Ras and cyclic AMP-dependent protein kinase.

3' splice site selection in dicot plant nuclei is position dependent.

The distal enhancer implicated in the developmental regulation of the tyrosine aminotransferase gene is bound by liver-specific and ubiquitous factors.

TFEC, a basic helix-loop-helix protein, forms heterodimers with TFE3 and inhibits TFE3-dependent transcription activation.

Exon switching and activation of stromal and embryonic fibroblast growth factor (FGF)-FGF receptor genes in prostate epithelial cells accompany stromal independence and malignancy.

Developmental differences in methylation of human Alu repeats.

Recognition DNA sequences of interferon regulatory factor 1 (IRF-1) and IRF-2, regulators of cell growth and the interferon system.

Identification and characterization of a new mammalian mitogen-activated protein kinase kinase, MKK2.

Tissue-specific alternative splicing of the Drosophila dopa decarboxylase gene is affected by heat shock.

A novel repression module, an extensive activation domain, and a bipartite nuclear localization signal defined in the immediate-early transcription factor Egr-1.

Uncoupling of initiation and reinitiation rates during HeLa RNA polymerase II transcription in vitro.

Centromeres of the fission yeast Schizosaccharomyces pombe are highly variable genetic loci.

A bipartite nuclear localization signal in the retinoblastoma gene product and its importance for biological activity.

A novel recognition motif for phosphatidylinositol 3-kinase binding mediates its association with the hepatocyte growth factor/scatter factor receptor.

Cooperative DNA binding of the human HoxB5 (Hox-2.1) protein is under redox regulation in vitro.

Guanine nucleotide exchange factor for eukaryotic translation initiation factor 2 in Saccharomyces cerevisiae: interactions between the essential subunits GCD2, GCD6, and GCD7 and the regulatory subunit GCN3.

Mechanism of developmental regulation of alpha pi, the chicken embryonic alpha-globin gene.

Identification of transcriptional activation and inhibitory domains in serum response factor (SRF) by using GAL4-SRF constructs.

Identification and characterization of a high-affinity interaction between v-Crk and tyrosine-phosphorylated paxillin in CT10-transformed fibroblasts.

Direct transcriptional stimulation of the ornithine decarboxylase gene by Fos in PC12 cells but not in fibroblasts.

Functional characterization of elements in a human U6 small nuclear RNA gene distal control region.

Molecular cloning, expression, and characterization of the human mitogen-activated protein kinase p44erk1.

CSE1 and CSE2, two new genes required for accurate mitotic chromosome segregation in Saccharomyces cerevisiae.

Evidence that the middle T antigen of polyomavirus interacts with the membrane skeleton.

E1A-mediated inhibition of myogenesis correlates with a direct physical interaction of E1A12S and basic helix-loop-helix proteins.

Yeast phenotype classifies mammalian protein kinase C cDNA mutants.

Regulation of the C/EBP-related gene gadd153 by glucose deprivation.

NPK1, a tobacco gene that encodes a protein with a domain homologous to yeast BCK1, STE11, and Byr2 protein kinases.

ADD1: a novel helix-loop-helix transcription factor associated with adipocyte determination and differentiation.

Identification of the immunophilins capable of mediating inhibition of signal transduction by cyclosporin A and FK506: roles of calcineurin binding and cellular location.

A dominant negative protein kinase C zeta subspecies blocks NF-kappa B activation.

Role of TFIIIA zinc fingers in vivo: analysis of single-finger function in developing Xenopus embryos.

Coexpression of two closely linked avian genes for purine nucleotide synthesis from a bidirectional promoter.

Identification of cis-acting regulatory elements controlling interleukin-4 gene expression in T cells: roles for NF-Y and NF-ATc.

Suppression of a defect in the 5' untranslated leader of mitochondrial COX3 mRNA by a mutation affecting an mRNA-specific translational activator protein.

Phenotypic effects of targeted mutations in the small subunit rRNA gene of Tetrahymena thermophila.

Yeast cells lacking 5'-->3' exoribonuclease 1 contain mRNA species that are poly(A) deficient and partially lack the 5' cap structure.

Role of gene order in developmental control of human gamma- and beta-globin gene expression.

Serum factors can modulate the developmental clock of gamma- to beta-globin gene switching in somatic cell hybrids.

Coupling of hormonal stimulation and transcription via the cyclic AMP-responsive factor CREB is rate limited by nuclear entry of protein kinase A.

TIF4631 and TIF4632: two yeast genes encoding the high-molecular-weight subunits of the cap-binding protein complex (eukaryotic initiation factor 4F) contain an RNA recognition motif-like sequence and carry out an essential function.

Ubiquitin pathway involvement in human lymphocyte gamma-irradiation-induced apoptosis.

An essential yeast protein, CBF5p, binds in vitro to centromeres and microtubules.

Lack of an initiator element is responsible for multiple transcriptional initiation sites of the TATA-less mouse thymidylate synthase promoter.

Transcription of the histone H5 gene is regulated by three differentiation-specific enhancers.

Localization of the E6-AP regions that direct human papillomavirus E6 binding, association with p53, and ubiquitination of associated proteins.

Activation of an imprinted Igf 2 gene in mouse somatic cell cultures.

Scanning and competition between AGs are involved in 3' splice site selection in mammalian introns.

Saccharomyces cerevisiae cdc15 mutants arrested at a late stage in anaphase are rescued by Xenopus cDNAs encoding N-ras or a protein with beta-transducin repeats.

Oncogenic activation of c-ABL by mutation within its last exon.

The protein encoded by a growth arrest-specific gene (gas6) is a new member of the vitamin K-dependent proteins related to protein S, a negative coregulator in the blood coagulation cascade.

Alpha 2-chimerin, an SH2-containing GTPase-activating protein for the ras-related protein p21rac derived by alternate splicing of the human n-chimerin gene, is selectively expressed in brain regions and testes.

Promoter elements determining weak expression of the GAL4 regulatory gene of Saccharomyces cerevisiae.

The immunosuppressant FK506 inhibits amino acid import in Saccharomyces cerevisiae.

Selective and synchronous activation of early-S-phase replicons of Ehrlich ascites cells.

The destabilizing elements in the coding region of c-fos mRNA are recognized as RNA.

Location and characterization of autonomously replicating sequences from chromosome VI of Saccharomyces cerevisiae.

The NF-kappa B and Sp1 motifs of the human immunodeficiency virus type 1 long terminal repeat function as novel thyroid hormone response elements.

Alteration of a yeast SH3 protein leads to conditional viability with defects in cytoskeletal and budding patterns.

Splicing mutants and their second-site suppressors at the dihydrofolate reductase locus in Chinese hamster ovary cells.

Translation of the yeast transcriptional activator GCN4 is stimulated by purine limitation: implications for activation of the protein kinase GCN2.

Expression of p60v-src in Saccharomyces cerevisiae results in elevation of p34CDC28 kinase activity and release of the dependence of DNA replication on mitosis.

A three-dimensional model of the Cdc2 protein kinase: localization of cyclin- and Suc1-binding regions and phosphorylation sites.

Activation of the HLA-DRA gene in primary human T lymphocytes: novel usage of TATA and the X and Y promoter elements.

Year: 1993, Volume: 13, Issue: 7

Year: 1993, Volume: 13, Issue: 6

Year: 1993, Volume: 13, Issue: 5

Year: 1993, Volume: 13, Issue: 4

Year: 1993, Volume: 13, Issue: 3

Year: 1993, Volume: 13, Issue: 2

Year: 1993, Volume: 13, Issue: 1

Year: 1992, Volume: 12

Year: 1991, Volume: 11

Year: 1990, Volume: 10

Year: 1989, Volume: 9

Year: 1988, Volume: 8

Year: 1987, Volume: 7

Year: 1986, Volume: 6

Year: 1985, Volume: 5

Year: 1984, Volume: 4

Year: 1983, Volume: 3

Year: 1982, Volume: 2

Year: 1981, Volume: 1

Promoter elements determining weak expression of the GAL4 regulatory gene of Saccharomyces cerevisiae.

Content Provider	PubMed Central
Author	Griggs, D. W. Johnston, M.
Abstract	The GAL4 gene of Saccharomyces cerevisiae (encoding the activator of transcription of the GAL genes) is poorly expressed and is repressed during growth on glucose. To determine the basis for its weak expression and to identify DNA sequences recognized by proteins that activate transcription of a gene that itself encodes an activator of transcription, we have analyzed GAL4 promoter structure. We show that the GAL4 promoter is about 90-fold weaker than the strong GAL1 promoter and at least 7-fold weaker than the feeble URA3 promoter and that this low level of GAL4 expression is primarily due to a weak promoter. By deletion mapping, the GAL4 promoter can be divided into three functional regions. Two of these regions contain positive elements; a distal region termed the UASGAL4 (upstream activation sequence) contains redundant elements that increase promoter function, and a central region termed the UESGAL4 (upstream essential sequence) is essential for even basal levels of GAL4 expression. The third element, an upstream repression sequence, mediates glucose repression of GAL4 expression and is located between the UES and the transcriptional start site. The UASGAL4 is unusual because it is not interchangable with UAS elements in other yeast promoters; it does not function as a UAS element when inserted in a CYC1 promoter, and a normally strong UAS functions poorly in place of UASGAL4 in the GAL4 promoter. Similarly, the UES element of GAL4 does not function as a TATA element in a test promoter, and consensus TATA elements do not function in place of UES elements in the GAL4 promoter. These results suggest that GAL4 contains a weak TATA-less promoter and that the proteins regulating expression of this regulatory gene may be novel and context specific.
Starting Page	4999
File Format	PDF
ISSN	10985549
e-ISSN	10985549
Journal	Molecular and Cellular Biology
Issue Number	8
Volume Number	13
Language	English
Publisher Date	1993-08-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Biology

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in