NDLI: Positive regulatory elements (HF-1a and HF-1b) and a novel negative regulatory element (HF-3) mediate ventricular muscle-specific expression of myosin light-chain 2-luciferase fusion genes in transgenic mice.

Please wait, while we are loading the content...

Year: 2016, Volume: 36

Year: 2015, Volume: 36

Year: 2015, Volume: 35

Year: 2014, Volume: 35

Year: 2014, Volume: 34

Year: 2013, Volume: 33

Year: 2012, Volume: 32

Year: 2011, Volume: 31

Year: 2010, Volume: 30

Year: 2009, Volume: 29

Year: 2008, Volume: 28

Year: 2007, Volume: 27

Year: 2006, Volume: 26

Year: 2005, Volume: 25

Year: 2004, Volume: 24

Year: 2003, Volume: 23

Year: 2002, Volume: 23

Year: 2002, Volume: 22

Year: 2001, Volume: 21

Year: 2000, Volume: 20

Year: 1999, Volume: 19

Year: 1998, Volume: 18

Year: 1997, Volume: 17

Year: 1996, Volume: 16

Year: 1995, Volume: 15

Year: 1994, Volume: 14

Year: 1994, Volume: 14, Issue: 12

Year: 1994, Volume: 14, Issue: 11

Year: 1994, Volume: 14, Issue: 10

Year: 1994, Volume: 14, Issue: 9

Year: 1994, Volume: 14, Issue: 8

Year: 1994, Volume: 14, Issue: 7

Year: 1994, Volume: 14, Issue: 6

Year: 1994, Volume: 14, Issue: 5

Year: 1994, Volume: 14, Issue: 4

Year: 1994, Volume: 14, Issue: 3

Year: 1994, Volume: 14, Issue: 2

Glucose-induced transcription of the insulin gene is mediated by factors required for beta-cell-type-specific expression.

A simple and efficient procedure for generating stable expression libraries by cDNA cloning in a retroviral vector.

Mechanisms of overlap formation in nonhomologous DNA end joining.

The mutant type 1 protein phosphatase encoded by glc7-1 from Saccharomyces cerevisiae fails to interact productively with the GAC1-encoded regulatory subunit.

Activation of Ras in vitro and in intact fibroblasts by the Vav guanine nucleotide exchange protein.

A unique palindromic element mediates gamma interferon induction of mig gene expression.

The B subunit of the DNA polymerase alpha-primase complex in Saccharomyces cerevisiae executes an essential function at the initial stage of DNA replication.

Two distinct transmembrane serine/threonine kinases from Drosophila melanogaster form an activin receptor complex.

Two different sequence elements within exon 4 are necessary for calcitonin-specific splicing of the human calcitonin/calcitonin gene-related peptide I pre-mRNA.

Restoration of telomeres in human papillomavirus-immortalized human anogenital epithelial cells.

Role of the histone amino termini in facilitated binding of a transcription factor, GAL4-AH, to nucleosome cores.

Prokaryotic expression cloning of a novel human tyrosine kinase.

Induction of the DNA-binding activity of c-jun/c-fos heterodimers by the hepatitis B virus transactivator pX.

Use of double-replacement gene targeting to replace the murine alpha-lactalbumin gene with its human counterpart in embryonic stem cells and mice.

Cloning and characterization of KNR4, a yeast gene involved in (1,3)-beta-glucan synthesis.

Retinoic acid represses Oct-3/4 gene expression through several retinoic acid-responsive elements located in the promoter-enhancer region.

Differential regulation of the c-myc oncogene promoter by the NF-kappa B rel family of transcription factors.

Interactions among three proteins that specifically activate translation of the mitochondrial COX3 mRNA in Saccharomyces cerevisiae.

Mutational activation of the STE5 gene product bypasses the requirement for G protein beta and gamma subunits in the yeast pheromone response pathway.

Anisomycin and rapamycin define an area upstream of p70/85S6k containing a bifurcation to histone H3-HMG-like protein phosphorylation and c-fos-c-jun induction.

Cdc42p GTPase is involved in controlling polarized cell growth in Schizosaccharomyces pombe.

Specific expression of the human CD4 gene in mature CD4+ CD8- and immature CD4+ CD8+ T cells and in macrophages of transgenic mice.

Ig alpha and Ig beta are functionally homologous to the signaling proteins of the T-cell receptor.

Identification of residues of the H-ras protein critical for functional interaction with guanine nucleotide exchange factors.

Identification of residues critical for Ras(17N) growth-inhibitory phenotype and for Ras interaction with guanine nucleotide exchange factors.

Characterization of a negative retinoic acid response element in the murine Oct4 promoter.

The gene encoding a major component of the lateral elements of synaptonemal complexes of the rat is related to X-linked lymphocyte-regulated genes.

Intracellular association of the protein product of the c-myc oncogene with the TATA-binding protein.

Activation of the granulocyte-macrophage colony-stimulating factor promoter in T cells requires cooperative binding of Elf-1 and AP-1 transcription factors.

Glucose repression of yeast mitochondrial transcription: kinetics of derepression and role of nuclear genes.

Physical and functional interaction between wild-type p53 and mdm2 proteins.

Pigment-cell-specific genes from fibroblasts are transactivated after chromosomal transfer into melanoma cells.

DNA repair defects associated with chromosomal translocation breaksite regions.

Activation of phospholipase C-gamma 1 through transfected platelet-derived growth factor receptor enhances interleukin 2 production upon antigen stimulation in a T-cell line.

Positive regulatory elements (HF-1a and HF-1b) and a novel negative regulatory element (HF-3) mediate ventricular muscle-specific expression of myosin light-chain 2-luciferase fusion genes in transgenic mice.

RFX1, a transactivator of hepatitis B virus enhancer I, belongs to a novel family of homodimeric and heterodimeric DNA-binding proteins.

HEN1 encodes a 20-kilodalton phosphoprotein that binds an extended E-box motif as a homodimer.

Preferred sequences for DNA recognition by the TAL1 helix-loop-helix proteins.

tRNA genes as transcriptional repressor elements.

Homologous, homeologous, and illegitimate repair of double-strand breaks during transformation of a wild-type strain and a rad52 mutant strain of Saccharomyces cerevisiae.

Two different types of double-strand breaks in Saccharomyces cerevisiae are repaired by similar RAD52-independent, nonhomologous recombination events.

Phenotypic rescue by a bovine transgene in a Cu/Zn superoxide dismutase-null mutant of Drosophila melanogaster.

Tyrosine phosphorylation of CD45 phosphotyrosine phosphatase by p50csk kinase creates a binding site for p56lck tyrosine kinase and activates the phosphatase.

HLA class I heavy-chain gene promoter elements mediating synergy between tumor necrosis factor and interferons.

The adenovirus E4-6/7 protein transactivates the E2 promoter by inducing dimerization of a heteromeric E2F complex.

Polypurine sequences within a downstream exon function as a splicing enhancer.

Xenopus gamma-crystallin gene expression: evidence that the gamma-crystallin gene family is transcribed in lens and nonlens tissues.

A factor induced by differentiation signals in cells of the macrophage lineage binds to the gamma interferon activation site.

Human T-cell leukemia virus type I Tax associates with and is negatively regulated by the NF-kappa B2 p100 gene product: implications for viral latency.

A novel cis-acting element controlling the rat CYP2D5 gene and requiring cooperativity between C/EBP beta and an Sp1 factor.

ATBF1, a multiple-homeodomain zinc finger protein, selectively down-regulates AT-rich elements of the human alpha-fetoprotein gene.

Purification of an oligo(dG).oligo(dC)-binding sea urchin nuclear protein, suGF1: a family of G-string factors involved in gene regulation during development.

suGF1 binds in the major groove of its oligo(dG).oligo(dC) recognition sequence and is excluded by a positioned nucleosome core.

High-resolution methylation analysis of the human hypoxanthine phosphoribosyltransferase gene 5' region on the active and inactive X chromosomes: correlation with binding sites for transcription factors.

Cloning and characterization of the cDNA encoding a novel human pre-B-cell colony-enhancing factor.

Nucleotide sequence of the yeast STE14 gene, which encodes farnesylcysteine carboxyl methyltransferase, and demonstration of its essential role in a-factor export.

Regulation and targeting of recombination in extrachromosomal substrates carrying immunoglobulin switch region sequences.

The carboxy-terminal region of mammalian HSP90 is required for its dimerization and function in vivo.

Nature and distribution of chromosomal intertwinings in Saccharomyces cerevisiae.

Human cancer cell lines express a negative transcriptional regulator of the interferon regulatory factor family of DNA binding proteins.

The Drosophila l(2)35Ba/nocA gene encodes a putative Zn finger protein involved in the development of the embryonic brain and the adult ocellar structures.

Structure and regulation of the human interferon regulatory factor 1 (IRF-1) and IRF-2 genes: implications for a gene network in the interferon system.

Cloning and characterization of centromeric DNA from Neurospora crassa.

Analysis of an origin of DNA amplification in Sciara coprophila by a novel three-dimensional gel method.

Year: 1994, Volume: 14, Issue: 1

Year: 1993, Volume: 13

Year: 1992, Volume: 12

Year: 1991, Volume: 11

Year: 1990, Volume: 10

Year: 1989, Volume: 9

Year: 1988, Volume: 8

Year: 1987, Volume: 7

Year: 1986, Volume: 6

Year: 1985, Volume: 5

Year: 1984, Volume: 4

Year: 1983, Volume: 3

Year: 1982, Volume: 2

Year: 1981, Volume: 1

Positive regulatory elements (HF-1a and HF-1b) and a novel negative regulatory element (HF-3) mediate ventricular muscle-specific expression of myosin light-chain 2-luciferase fusion genes in transgenic mice.

Content Provider	PubMed Central
Author	Lee, K. J. Hickey, R. Zhu, H. Chien, K. R.
Abstract	The cardiac myosin light-chain 2v (MLC-2v) gene has served as a model system to identify the pathways which restrict the expression of cardiac muscle genes to particular chambers of the heart during cardiogenesis. To identify the critical cis regulatory elements which mediate ventricular chamber-specific expression of the MLC-2v gene in the in vivo context, a series of transgenic mice which harbor mutations in putative MLC-2 cis regulatory elements in a 250-bp MLC-2-luciferase fusion gene which is expressed in a ventricular chamber-specific fashion in transgenic mice were generated. These studies demonstrate that both components of HF-1 (HF-1a and HF-1b/MEF-2) are required to maintain ventricular chamber-specific expression and function as positive regulatory elements. Mutations in another conserved element (HF-2) are without statistically significant effect on ventricular chamber expression. Transgenics harboring mutations in the E-box site also displayed significant upregulation of reporter activity in the soleus, gastrocnemius, and uterus, with a borderline effect on expression in liver. Mutations in another conserved element (HF-3) result in a marked (> 75-fold) upregulation of the luciferase reporter activity in the soleus muscle of multiple independent or transgenic founders. Since the HF-3 mutations appeared to have only a marginal effect on luciferase reporter activity in liver tissue, HF-3 appears to function as a novel negative regulatory element to primarily suppress expression in muscle tissues. Thus, a combination of positive (HF-1a/HF-1b) and negative (E-box and HF-3) regulatory elements appear to be required to maintain ventricular chamber-specific expression in the in vivo context.
Starting Page	1220
File Format	PDF
ISSN	10985549
e-ISSN	10985549
Journal	Molecular and Cellular Biology
Issue Number	2
Volume Number	14
Language	English
Publisher Date	1994-02-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Biology

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in