NDLI: Morphological analysis of the antimicrobial action of nitric oxide on Gram-negative pathogens using atomic force microscopy

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Morphological analysis of the antimicrobial action of nitric oxide on Gram-negative pathogens using atomic force microscopy

Content Provider	PubMed Central
Author	Deupree, Susan M. Schoenfisch, Mark H.
Abstract	Atomic force microscopy (AFM) was used to study the morphological changes of two Gram-negative pathogens, Pseudomonas aeruginosa and Escherichia coli, after exposure to nitric oxide (NO). The time-dependent effects of NO released from a xerogel coating and the concentration-dependent effects rendered by a small-molecule that releases NO in a bolus were examined and compared. Bacteria exhibited irregular and degraded exteriors. With NO-releasing surfaces, an increase in surface debris and disorganized adhesion patterns were observed compared to controls. Analysis of cell surface topography revealed that increasing membrane roughness correlated with higher doses of NO. At a lower total dose, NO delivered via a bolus resulted in greater membrane roughness than NO released from a surface via a sustained flux. At sub-inhibitory levels, treatment with amoxicillin, an antibiotic known to compromise the integrity of the cell wall, led to morphologies resembling those resulting from NO treatment. Our observations indicate that cell envelope deterioration is a visible consequence of NO-exposure for both Gram-negative species studied.
Related Links	http://dx.doi.org/10.1016/j.actbio.2009.01.025
Ending Page	1415
Page Count	11
Starting Page	1405
File Format	PDF
ISSN	18787568
e-ISSN	18787568
Journal	Acta biomaterialia
Issue Number	5
Volume Number	5
Language	English
Publisher Date	2009-06-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Biomaterials Molecular Biology Biochemistry Biomedical Engineering Biotechnology

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