NDLI: The Excision Proteins of CTnDOT Positively Regulate the Transfer Operon

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The Excision Proteins of CTnDOT Positively Regulate the Transfer Operon

Content Provider	PubMed Central
Author	Keeton, Carolyn M. Park, Jiyeon Wang, Gui-rong Hopp, Crystal M. Shoemaker, Nadja B. Gardner, Jeffrey F. Salyers, Abigail A.
Abstract	The Bacteroides conjugative transposon, CTnDOT, is an integrated conjugative element (ICE), found in many human colonic Bacteroides spp. strains. It has a complex regulatory system for both excision from the chromosome and transfer and mobilization into a new host. It was previously shown that a cloned DNA segment encoding the xis2c, xis2d, orf3, and exc genes was required for tetracycline dependent activation of the Ptra promoter. The Xis2c and Xis2d proteins are required for excision while the Exc protein stimulates excision. We report here that neither the Orf3 nor the Exc proteins are involved in activation of the Ptra promoter. Deletion analysis and electromobility shift assays showed that the Xis2c and Xis2d proteins bind to the Ptra promoter to activate the tra operon. Thus, the recombination directionality factors of CTnDOT excision also function as activator proteins of the Ptra promoter.
Related Links	http://dx.doi.org/10.1016/j.plasmid.2012.12.001
Ending Page	179
Page Count	8
Starting Page	172
File Format	PDF
ISSN	0147619X
e-ISSN	10959890
Journal	Plasmid
Issue Number	2
Volume Number	69
Language	English
Publisher Date	2013-03-01
Access Restriction	Open
Subject Keyword	Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Molecular Biology

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