NDLI: Fabricating Reversible and Regenerable Raman-active Substrate with Biomolecule-controlled DNA Nanomachine

Content Provider	PubMed Central
Author	Zheng, Jing Jiao, Anli Yang, Ronghua Li, Huimin Li, Jishan Shi, Muling Cheng, Ma Jiang, Ying Deng, Li Tan, Weihong
Abstract	A DNA configuration switch was designed to fabricate a reversible and regenerable Raman-active substrate. The substrate is composed of an Au film and hairpin-shaped DNA strand (hot spot generation probes, HSGPs) labeled with dye-functionalized silver nanoparticle (AgNP). Another ssDNA that recognizes a specific trigger was used as an antenna. The HSGPs are immobilized on the Au film to draw the dye-functionalized AgNPs close to the Au surface and create an intense electromagnetic field. Hybridization of HSGP with the two arm segments of the antenna forms a triplex-stem structure to separate the dye-functionalized AgNP from the Au surface and cause a quenching of the Raman signal. Interaction with its trigger leads to release of the antenna from the triplex-stem structure, and the hairpin structure of the HSGP is restored, thereby creating an effective “off” to “on” state of the Raman signal. Nucleic acid sequence associated with the HIV-1 U5 long terminal repeat sequences and ATP are used as the triggers. The substrate shows excellent reversibility, reproducibility and controllability of SERS effects, which are significant requirements for practical SERS sensor applications.
Related Links	http://dx.doi.org/10.1021/ja308875r
Ending Page	19960
Page Count	4
Starting Page	19957
File Format	PDF
ISSN	00027863
e-ISSN	15205126
Journal	Journal of the American Chemical Society
Issue Number	49
Volume Number	134
Language	English
Publisher Date	2012-12-12
Access Restriction	Open
Subject Keyword	Colloid and Surface Chemistry Biochemistry Chemistry(all) Catalysis Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Chemistry Colloid and Surface Chemistry Biochemistry Catalysis

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