NDLI: Roles of Genes 44, 50, and 51 in Regulating Gene Expression and Host Takeover during Infection of Bacillus subtilis by Bacteriophage SPO1

Content Provider	PubMed Central
Author	Sampath, Aruna Stewart, Charles R.
Copyright Year	2004
Abstract	We show that the products of SPO1 genes 44, 50, and 51 are required for the normal transition from early to middle gene expression during infection of Bacillus subtilis by bacteriophage SPO1; that they are also required for control of the shutoff of host DNA, RNA, and protein synthesis; and that their effects on host shutoff could be accounted for by their effects on the regulation of gene expression. These three gene products had four distinguishable effects in regulating SPO1 gene expression: (i) gp44-50-51 acted to restrain expression of all SPO1 genes tested, (ii) gp44 and/or gp50-51 caused additional specific repression of immediate-early genes, (iii) gp44 and/or gp50-51 stimulated expression of middle genes, and (iv) gp44 and/or gp50-51 stimulated expression of some delayed-early genes. Shutoff of immediate-early gene expression also required the activity of gp28, the middle-gene-specific sigma factor. Shutoff of host RNA and protein synthesis was accelerated by either the 44− single mutant or the 50−51− double mutant and more so by the 44−50−51− triple mutant. Shutoff of host DNA synthesis was accelerated by the mutants early in infection but delayed by the 44−50−51− triple mutant at later times. Although gp50 is a very small protein, consisting almost entirely of an apparent membrane-spanning domain, it contributed significantly to each activity tested. We identify SPO1 genes 41 to 51 and 53 to 60 as immediate-early genes; genes 27, 28, and 37 to 40 as delayed-early genes; and gene 52 as a middle gene.
Related Links	http://dx.doi.org/10.1128/jb.186.6.1785-1792.2004
Ending Page	1792
Page Count	8
Starting Page	1785
File Format	PDF
ISSN	00219193
e-ISSN	10985530
Journal	Journal of Bacteriology
Issue Number	6
Volume Number	186
Language	English
Publisher	American Society for Microbiology
Publisher Date	2004-03-15
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Molecular Biology Microbiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Microbiology

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