NDLI: Association of inducible nitric oxide synthetase genotype and Helicobacter pylori infection gastric cancer risk may be due to faulty primer design

Content Provider	PubMed Central
Author	Abadi, Amin Talebi Bezmin Kusters, Johannes G.
Copyright Year	2013
Abstract	Rafiei et al recently described an association between the presence of the C150T polymorphism of the inducible nitric oxide synthase (iNOS) gene and Helicobacter pylori (H. pylori) induced gastric cancer. When we used primer-BLAST to find the polymerase chain reaction (PCR) product that would be generated by the primers used by these authors no products against any of the sequences present in the GenBank database were found. Further analysis of the iNOS sequences present in the GenBank suggest that the result from their study might come from a faulty primer design and may thus represent an artifact. Alternatively they may be correct and have identified a truly interesting explanation for the mechanism whereby H. pylori induces gastric cancer but some additional experiments would be in order to exclude the possibility of a PCR artifact.
Related Links	http://dx.doi.org/10.3748/wjg.v19.i3.429
Starting Page	429
File Format	PDF
ISSN	10079327
Journal	World Journal of Gastroenterology : WJG
Issue Number	3
Volume Number	19
Language	English
Publisher	Baishideng Publishing Group Co., Limited
Publisher Date	2013-01-01
Access Restriction	Open
Rights Holder	Baishideng Publishing Group Co., Limited
Subject Keyword	Gastroenterology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Gastroenterology

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Association of inducible nitric oxide synthetase genotype and Helicobacter pylori infection gastric cancer risk may be due to faulty primer design