NDLI: Characterization of BreR Interaction with the Bile Response Promoters breAB and breR in Vibrio cholerae

Content Provider	PubMed Central
Author	Cerda-maira, Francisca A. Gabriela, Kovacikova Jude, Brooke A. Skorupski, Karen Taylor, Ronald K.
Copyright Year	2013
Abstract	The Vibrio cholerae BreR protein is a transcriptional repressor of the breAB efflux system operon, which encodes proteins involved in bile resistance. In a previous study (F. A. Cerda-Maira, C. S. Ringelberg, and R. K. Taylor, J. Bacteriol. 190:7441–7452, 2008), we used gel mobility shift assays to determine that BreR binds at two independent binding sites at the breAB promoter and a single site at its own promoter. Here it is shown, by DNase I footprinting and site-directed mutagenesis, that BreR is able to bind at a distal and a proximal site in the breAB promoter. However, only one of these sites, the proximal 29-bp site, is necessary for BreR-mediated transcriptional repression of breAB expression. In addition, it was determined that BreR represses its own expression by recognizing a 28-bp site at the breR promoter. These sites comprise regions of dyad symmetry within which residues critical for BreR function could be identified. The BreR consensus sequence AANGTANAC-N6-GTNTACNTT overlaps the −35 region at both promoters, implying that the repression of gene expression is achieved by interfering with RNA polymerase binding at these promoters.
Related Links	http://dx.doi.org/10.1128/jb.02008-12
Ending Page	317
Page Count	11
Starting Page	307
File Format	PDF
ISSN	00219193
e-ISSN	10985530
Journal	Journal of Bacteriology
Issue Number	2
Volume Number	195
Language	English
Publisher	American Society for Microbiology
Publisher Date	2013-01-15
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Molecular Biology Microbiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Microbiology

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The Bile Response Repressor BreR Regulates Expression of the Vibrio cholerae breAB Efflux System Operon▿ †

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