NDLI: The cell biology of peritrichous flagella in Bacillus subtilis

Content Provider	PubMed Central
Author	Guttenplan, Sarah B. Shaw, Sidney Kearns, Daniel B.
Abstract	Bacterial flagella are highly conserved molecular machines that have been extensively studied for assembly, function, and gene regulation. Less studied is how and why bacteria differ based on the number and arrangement of the flagella they synthesize. Here we explore the cell biology of peritrichous flagella in the model bacterium Bacillus subtilis by fluorescently labeling flagellar basal bodies, hooks, and filaments. We find that the average B. subtilis cell assembles approximately 26 flagellar basal bodies and we show that basal body number is controlled by the protein of unknown function SwrA. Basal bodies are assembled rapidly (< 5 minutes) but the assembly of flagella capable of supporting motility (> 40 minutes) is rate limited by filament polymerization. We find that basal bodies are not positioned randomly on the cell surface. Rather, basal bodies occupy a grid-like pattern organized symmetrically around the midcell and that flagella are discouraged at the poles. Basal body position is genetically determined by FlhF and FlhG homologs to control spatial patterning differently from what is seen in bacteria with polar flagella. Finally, spatial control of flagella in B. subtilis seems more relevant to the inheritance of flagella in individual cells than the motile behavior of populations.
Related Links	http://dx.doi.org/10.1111/mmi.12103
Ending Page	229
Page Count	19
Starting Page	211
File Format	PDF
ISSN	0950382X
e-ISSN	13652958
Journal	Molecular microbiology
Issue Number	1
Volume Number	87
Language	English
Publisher Date	2013-01-01
Access Restriction	Open
Subject Keyword	Molecular Biology Microbiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Microbiology

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