NDLI: MET Is Required for the Maximal Action of 20-Hydroxyecdysone during Bombyx Metamorphosis

Content Provider	PubMed Central
Author	Guo, Enen He, Qianyu Liu, Shumin Tian, Ling Sheng, Zhentao Peng, Qin Guan, Jingmin Shi, Mingan Li, Kang Gilbert, Lawrence I. Wang, Jian Cao, Yang Li, Sheng
Editor	Picard, Didier
Copyright Year	2012
Abstract	Little is known about how the putative juvenile hormone (JH) receptor, the bHLH-PAS transcription factor MET, is involved in 20-hydroxyecdysone (20E; the molting hormone) action. Here we report that two MET proteins found in the silkworm, Bombyx mori, participate in 20E signal transduction. Met is 20E responsive and its expression peaks during molting and pupation, when the 20E titer is high. As found with results from RNAi knockdown of EcR-USP (the ecdysone receptor genes), RNAi knockdown of Met at the early wandering stage disrupts the 20E-triggered transcriptional cascade, preventing tissue remodeling (including autophagy, apoptosis and destruction of larval tissues and generation of adult structures) and causing lethality during the larval-pupal transition. MET physically interacts with EcR-USP. Moreover, MET, EcR-USP and the 20E-response element (EcRE) form a protein-DNA complex, implying that MET might modulate 20E-induced gene transcription by interacting with EcR-USP. In conclusion, the 20E induction of MET is required for the maximal action of 20E during Bombyx metamorphosis.
Related Links	http://dx.doi.org/10.1371/journal.pone.0053256
Starting Page	53256
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	12
Volume Number	7
Language	English
Publisher	Public Library of Science
Publisher Date	2012-12-01
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Cysteine 904 Is Required for Maximal Insulin Degrading Enzyme Activity and Polyanion Activation

Identification of 20-Hydroxyecdysone Late-Response Genes in the Chitin Biosynthesis Pathway

Erbb2 Is Required for Cardiac Atrial Electrical Activity during Development

20-Hydroxyecdysone (20E) Primary Response Gene E93 Modulates 20E Signaling to Promote Bombyx Larval-Pupal Metamorphosis

Loss of c-Met Disrupts Gene Expression Program Required for G2/M Progression during Liver Regeneration in Mice

HDAC Activity Is Required during Xenopus Tail Regeneration

PolyADP-Ribosylation Is Required for Pronuclear Fusion during Postfertilization in Mice

The Mych Gene Is Required for Neural Crest Survival during Zebrafish Development

DAAM1 Is a Formin Required for Centrosome Re-Orientation during Cell Migration

MET Is Required for the Maximal Action of 20-Hydroxyecdysone during Bombyx Metamorphosis

Similar Documents

Cysteine 904 Is Required for Maximal Insulin Degrading Enzyme Activity and Polyanion Activation

Identification of 20-Hydroxyecdysone Late-Response Genes in the Chitin Biosynthesis Pathway

Erbb2 Is Required for Cardiac Atrial Electrical Activity during Development

20-Hydroxyecdysone (20E) Primary Response Gene E93 Modulates 20E Signaling to Promote Bombyx Larval-Pupal Metamorphosis

Loss of c-Met Disrupts Gene Expression Program Required for G2/M Progression during Liver Regeneration in Mice

HDAC Activity Is Required during Xenopus Tail Regeneration

PolyADP-Ribosylation Is Required for Pronuclear Fusion during Postfertilization in Mice

The Mych Gene Is Required for Neural Crest Survival during Zebrafish Development

DAAM1 Is a Formin Required for Centrosome Re-Orientation during Cell Migration

MET Is Required for the Maximal Action of 20-Hydroxyecdysone during Bombyx Metamorphosis