NDLI: Melanoma cell invasiveness is promoted at least in part by the Epidermal Growth Factor-like repeats of Tenascin-C

Content Provider	PubMed Central
Author	Grahovac, Jelena Becker, Dorothea Wells, Alan
Abstract	Tenascin-C (TNC), overexpressed in invasive growths, has been implicated in progression of melanoma but the source and function of this molecule are not well defined. We found TNC expression at the front of invading melanoma cells, and that adding TNC to matrices enhances individual melanoma cell migration. As TNC is a multidomain protein, we examined the role of the TNC EGF-like repeats (EGFL) as these activate motogenic signaling cascades. We overexpressed a TNC fragment containing the assembly and EGFL domains of TNC (TNCEGFL). TNCEGFL-expressing melanoma cells had lower speed and persistence in 2D migration assays due to a shift in the adhesion-contractility balance, as expression of TNCEGFL delayed melanoma cell attachment and spreading. The less adhesive phenotype was due, in part, to increased ROCK signaling concomitant with MLC2 and MYPT phosphorylation. Inhibition of ROCK activity, which drives transcellular contractility, restored adhesion of TNCEGFL expressing melanoma cells and increased their migration in 2D. In contrast to the diminished migration in 2D, TNCEGFL-expressing melanoma cells had higher invasive potential in Matrigel invasion assays, with cells expressing TNCEGFL having amoeboid morphology. Our findings suggest that melanoma-derived TNC EGFL play a role in melanoma invasion by modulating ROCK signaling and cell migration.
Related Links	http://dx.doi.org/10.1038/jid.2012.263
Ending Page	220
Page Count	11
Starting Page	210
File Format	PDF
ISSN	0022202X
e-ISSN	15231747
Journal	The Journal of investigative dermatology
Issue Number	1
Volume Number	133
Language	English
Publisher Date	2013-01-01
Access Restriction	Open
Subject Keyword	Cell Biology Biochemistry Molecular Biology Dermatology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology Dermatology

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