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In situ mass spectrometry of autoimmune liver diseases

Content Provider	PubMed Central
Author	Bowlus, Christopher L. Seeley, Erin H. Roder, Joanna Grigorieva, Julia Roder, Heinrich Caprioli, Richard M. Gershwin, Meric
Copyright Year	2011
Abstract	Primary biliary cirrhosis (PBC), primary sclerosing cholangitis (PSC) and autoimmune hepatitis (AIH) are the major forms of autoimmune liver diseases each characterized by the destruction of a specific liver cell type and the presence of differing auto-antibodies. We took a proteomic approach utilizing in situ matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) to obtain profiles directly from liver samples of patients with PBC, PSC, AIH and controls. The ability to precisely localize the region for acquisition of MALDI MS allowed us to obtain profiles from bile ducts, inflammatory infiltrates and hepatocytes from each biopsy sample. Analysis tools developed to identify peaks and compare peaks across diseases and cell types were used to develop models to classify the samples. Using an initial set of testing samples from PBC patients and controls, we identified unique peaks present in bile ducts, inflammatory infiltrates and hepatocytes that could classify samples in a validation cohort with 88–91% accuracy. Interestingly, profiles of PSC and AIH did not differ significantly from PBC. Identification of proteins in these peaks may represent novel autoantigens or effector molecules. These findings illustrate the potential of a proteomic approach to autoimmune diseases with in situ MALDI MS.
Related Links	http://dx.doi.org/10.1038/cmi.2010.72
Ending Page	242
Page Count	6
Starting Page	237
File Format	PDF
ISSN	16727681
e-ISSN	20420226
Journal	Cellular and Molecular Immunology
Issue Number	3
Volume Number	8
Language	English
Publisher	Nature Publishing Group
Publisher Date	2011-05-01
Access Restriction	Open
Rights Holder	Nature Publishing Group
Subject Keyword	Immunology Immunology and Allergy Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Immunology and Allergy Immunology

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