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Identification of B Cell and T Cell Epitopes Using Synthetic Peptide Combinatorial Libraries
| Content Provider | PubMed Central |
|---|---|
| Author | Pinilla, Clemencia Appel, Jon R. Valeria, Judkowski Houghten, Richard A. |
| Abstract | This unit presents a combinatorial library method that consists of the synthesis and screening of mixture-based synthetic combinatorial libraries of peptide molecules. The protocols employ peptide libraries to identify peptides recognized by mAbs and T cells. The first protocol uses a positional scanning peptide library made up of hexapeptides to identify antigenic determinants recognized by mAbs. The 120 mixtures in the hexapeptide library are tested for their inhibitory activity in a competitive ELISA. The second protocol uses a decapeptide library to identify T cell peptide ligands. The 200 mixtures of the decapeptide library are tested for their ability to induce T cell activation. Support protocols cover optimization of the assay conditions for each mAb or T cell, to achieve the best level of sensitivity and reproducibility, and preparation of a hexapeptide library, along with deconvolution approaches. |
| Related Links | http://dx.doi.org/10.1002/0471142735.im0905s99 |
| File Format | |
| ISSN | 1934368X |
| e-ISSN | 1934368X |
| Journal | Current protocols in immunology / edited by John E. Coligan ... [et al.] |
| Volume Number | CHAPTER |
| Language | English |
| Publisher Date | 2012-11-01 |
| Access Restriction | Open |
| Subject Keyword | Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Immunology |
