NDLI: Germline Ablation of VGF Increases Lipolysis in White Adipose Tissue

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Germline Ablation of VGF Increases Lipolysis in White Adipose Tissue

Content Provider	PubMed Central
Author	Samira, Fargali Scherer, Thomas Shin, Andrew C. Sadahiro, Masato Buettner, Christoph Salton, Stephen R.
Abstract	Targeted deletion of VGF, a neuronal and endocrine secreted protein and neuropeptide precursor, produces a lean, hypermetabolic mouse that is resistant to diet-, lesion-, and genetically-induced obesity and diabetes. We hypothesized that increased sympathetic nervous system activity in Vgf−/Vgf− knockout mice is responsible for increased energy expenditure and decreased fat storage, and that increased beta-adrenergic receptor stimulation induces lipolysis in white adipose tissue (WAT) of Vgf−/Vgf− mice. We found that fat mass was markedly reduced in Vgf−/Vgf− mice. Within knockout WAT, phosphorylation of protein kinase A (PKA) substrate increased in males and females, phosphorylation of hormone sensitive lipase (HSL) (Ser563) increased in females, and levels of adipose triglyceride lipase (ATGL), comparative gene identification-58 (CGI-58), and phospho-perilipin, were higher in male Vgf−/Vgf− WAT compared to wild type, consistent with increased lipolysis. The phosphorylation of AMP-activated protein kinase (AMPK) (Thr172) and levels of the AMPK kinase, transforming growth factor β-activated kinase 1 (TAK-1), were decreased. This was associated with a decrease in HSL Ser565 phosphorylation, the site phosphorylated by AMPK, in both male and female Vgf−/Vgf− WAT. No significant differences in phosphorylation of cAMP response element binding protein (CREB) or the p42/44 mitogen-activated protein kinase (MAPK) were noted. Despite this evidence supporting increased cAMP signaling and lipolysis, lipogenesis as assessed by fatty acid synthase (FAS) protein expression and phosphorylated acetyl-CoA carboxylase (pACC) was not decreased. Our data suggest that the VGF precursor or selected VGF-derived peptides dampen sympathetic outflow pathway activity to WAT to regulate fat storage and lipolysis.
Related Links	http://dx.doi.org/10.1530/joe-12-0172
Ending Page	322
Page Count	10
Starting Page	313
File Format	PDF
ISSN	00220795
e-ISSN	14796805
Journal	The Journal of endocrinology
Issue Number	2
Volume Number	215
Language	English
Publisher Date	2012-11-01
Access Restriction	Open
Subject Keyword	Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Endocrinology, Diabetes and Metabolism Endocrinology

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