NDLI: Uptake of extracellular double-stranded RNA by SID-2

Content Provider	PubMed Central
Author	Mcewan, Deborah L. Weisman, Alexandra S. Hunter, Craig P.
Copyright Year	2012
Abstract	Ingested dsRNAs trigger RNA interference (RNAi) in many invertebrates including the nematode Caenorhabditis elegans. Here we show that the C. elegans apical intestinal membrane protein SID-2 is required in C. elegans for the import of ingested dsRNA and, when expressed in Drosophila S2 cells, SID-2 enables the uptake of dsRNAs. SID-2-dependent dsRNA transport requires an acidic extracellular environment and is selective for dsRNAs with at least 50 base pairs. Through structure-function analysis, we identify several SID-2 regions required for this activity including three extracellular, positively-charged, histidines. Finally, we find that SID-2-dependent transport is inhibited by drugs that interfere with vesicle transport. Therefore, we propose that environmental dsRNAs are imported from the acidic intestinal lumen by SID-2 via endocytosis and are released from internalized vesicles in a secondary step mediated by the dsRNA-channel SID-1. Similar multistep mechanisms may underlie the widespread observations of environmental RNAi.
Related Links	http://dx.doi.org/10.1016/j.molcel.2012.07.014
Ending Page	754
Page Count	9
Starting Page	746
File Format	PDF
ISSN	10972765
e-ISSN	10974164
Journal	Molecular cell
Issue Number	5
Volume Number	47
Language	English
Publisher Date	2012-09-01
Access Restriction	Open
Subject Keyword	Cell Biology Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Biology

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Systemic RNA Interference Deficiency-1 (SID-1) Extracellular Domain Selectively Binds Long Double-stranded RNA and Is Required for RNA Transport by SID-1

Systemic RNA Interference Deficiency-1 (SID-1) Extracellular Domain Selectively Binds Long Double-stranded RNA and Is Required for RNA Transport by SID-1.

The SID-1 double-stranded RNA transporter is not selective for dsRNA length

Gene induction by interferons and double-stranded RNA: selective inhibition by 2-aminopurine.

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Tissue-specific overexpression of the double-stranded RNA transporter SID-1 limits lifespan in C. elegans

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Uptake of extracellular double-stranded RNA by SID-2

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Systemic RNA Interference Deficiency-1 (SID-1) Extracellular Domain Selectively Binds Long Double-stranded RNA and Is Required for RNA Transport by SID-1

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