NDLI: Effects of neuronal nitric oxide synthase inhibition on microvascular and contractile function in skeletal muscle of aged rats

Content Provider	PubMed Central
Author	Hirai, Daniel M. Copp, Steven W. Holdsworth, Clark T. Ferguson, Scott K. Musch, Timothy I. Poole, David C.
Copyright Year	2012
Abstract	Advanced age is associated with derangements in skeletal muscle microvascular function during the transition from rest to contractions. We tested the hypothesis that, contrary to what was reported previously in young rats, selective neuronal nitric oxide (NO) synthase (nNOS) inhibition would result in attenuated or absent alterations in skeletal muscle microvascular oxygenation (Po 2 mv), which reflects the matching between muscle O2 delivery and utilization, following the onset of contractions in old rats. Spinotrapezius muscle blood flow (radiolabeled microspheres), Po 2 mv (phosphorescence quenching), O2 utilization (V̇o 2; Fick calculation), and submaximal force production were measured at rest and following the onset of contractions in anesthetized old male Fischer 344 × Brown Norway rats (27 to 28 mo) pre- and postselective nNOS inhibition (2.1 μmol/kg S-methyl-l-thiocitrulline; SMTC). At rest, SMTC had no effects on muscle blood flow (P > 0.05) but reduced V̇o 2 by ∼23% (P < 0.05), which elevated basal Po 2 mv by ∼18% (P < 0.05). During contractions, steady-state muscle blood flow, V̇o 2, Po 2 mv, and force production were not altered after SMTC (P > 0.05 for all). The overall Po 2 mv dynamics following onset of contractions was also unaffected by SMTC (mean response time: pre, 19.7 ± 1.5; and post, 20.0 ± 2.0 s; P > 0.05). These results indicate that the locus of nNOS-derived NO control in skeletal muscle depends on age and metabolic rate (i.e., rest vs. contractions). Alterations in nNOS-mediated regulation of contracting skeletal muscle microvascular function with aging may contribute to poor exercise capacity in this population.
Related Links	http://dx.doi.org/10.1152/ajpheart.00477.2012
Starting Page	1076
File Format	PDF
ISSN	15221539
e-ISSN	15221539
Journal	American Journal of Physiology - Heart and Circulatory Physiology
Issue Number	8
Volume Number	303
Language	English
Publisher	American Physiological Society
Publisher Date	2012-10-15
Access Restriction	Open
Rights Holder	American Physiological Society
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Physiology Physiology (medical) Cardiology and Cardiovascular Medicine

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