NDLI: Exploring the DNA-binding specificities of zinc fingers with DNA microarrays

Content Provider	PubMed Central
Author	Bulyk, Martha L. Huang, Xiaohua Choo, Yen Church, George M.
Copyright Year	2001
Abstract	A key step in the regulation of networks that control gene expression is the sequence-specific binding of transcription factors to their DNA recognition sites. A more complete understanding of these DNA–protein interactions will permit a more comprehensive and quantitative mapping of the regulatory pathways within cells, as well as a deeper understanding of the potential functions of individual genes regulated by newly identified DNA-binding sites. Here we describe a DNA microarray-based method to characterize sequence-specific DNA recognition by zinc-finger proteins. A phage display library, prepared by randomizing critical amino acid residues in the second of three fingers of the mouse Zif268 domain, provided a rich source of zinc-finger proteins with variant DNA-binding specificities. Microarrays containing all possible 3-bp binding sites for the variable zinc fingers permitted the quantitation of the binding site preferences of the entire library, pools of zinc fingers corresponding to different rounds of selection from this library, as well as individual Zif268 variants that were isolated from the library by using specific DNA sequences. The results demonstrate the feasibility of using DNA microarrays for genome-wide identification of putative transcription factor-binding sites.
Related Links	http://dx.doi.org/10.1073/pnas.111163698
Ending Page	7163
Page Count	6
Starting Page	7158
File Format	PDF
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	13
Volume Number	98
Language	English
Publisher	The National Academy of Sciences
Publisher Date	2001-06-19
Access Restriction	Open
Rights Holder	The National Academy of Sciences
Subject Keyword	General Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Exploring the DNA-binding specificities of zinc fingers with DNA microarrays.

Zinc fingers, zinc clusters, and zinc twists in DNA-binding protein domains.

Rapid Analysis of the DNA Binding Specificities of Transcription Factors with DNA Microarrays

Analysis of the Sequence Specificities of DNA Binding Proteins with Protein Binding Microarrays

Selection of DNA binding sites for zinc fingers using rationally randomized DNA reveals coded interactions.

DNA binding specificities of the long zinc-finger recombination protein PRDM9

Universal protein binding microarrays for the comprehensive characterization of the DNA binding specificities of transcription factors

Quantitative modeling of transcription factor binding specificities using DNA shape

Toward a code for the interactions of zinc fingers with DNA: Selection of randomized fingers displayed on phage

Exploring the DNA-binding specificities of zinc fingers with DNA microarrays

Similar Documents

Exploring the DNA-binding specificities of zinc fingers with DNA microarrays.

Zinc fingers, zinc clusters, and zinc twists in DNA-binding protein domains.

Rapid Analysis of the DNA Binding Specificities of Transcription Factors with DNA Microarrays

Analysis of the Sequence Specificities of DNA Binding Proteins with Protein Binding Microarrays

Selection of DNA binding sites for zinc fingers using rationally randomized DNA reveals coded interactions.

DNA binding specificities of the long zinc-finger recombination protein PRDM9

Universal protein binding microarrays for the comprehensive characterization of the DNA binding specificities of transcription factors

Quantitative modeling of transcription factor binding specificities using DNA shape

Toward a code for the interactions of zinc fingers with DNA: Selection of randomized fingers displayed on phage

Exploring the DNA-binding specificities of zinc fingers with DNA microarrays