NDLI: Exploring the DNA mimicry of the Ocr protein of phage T7

Content Provider	PubMed Central
Author	Roberts, Gareth A. Stephanou, Augoustinos S. Kanwar, Nisha Dawson, Angela Cooper, Laurie P. Chen, Kai Nutley, Margaret Cooper, Alan Blakely, Garry W. Dryden, David T. F.
Copyright Year	2012
Abstract	DNA mimic proteins have evolved to control DNA-binding proteins by competing with the target DNA for binding to the protein. The Ocr protein of bacteriophage T7 is the most studied DNA mimic and functions to block the DNA-binding groove of Type I DNA restriction/modification enzymes. This binding prevents the enzyme from cleaving invading phage DNA. Each 116 amino acid monomer of the Ocr dimer has an unusual amino acid composition with 34 negatively charged side chains but only 6 positively charged side chains. Extensive mutagenesis of the charges of Ocr revealed a regression of Ocr activity from wild-type activity to partial activity then to variants inactive in antirestriction but deleterious for cell viability and lastly to totally inactive variants with no deleterious effect on cell viability. Throughout the mutagenesis the Ocr mutant proteins retained their folding. Our results show that the extreme bias in charged amino acids is not necessary for antirestriction activity but that less charged variants can affect cell viability by leading to restriction proficient but modification deficient cell phenotypes.
Related Links	http://dx.doi.org/10.1093/nar/gks516
Starting Page	8129
File Format	PDF
ISSN	13624962
e-ISSN	13624962
Journal	Nucleic Acids Research
Issue Number	16
Volume Number	40
Language	English
Publisher	Oxford University Press
Publisher Date	2012-09-01
Access Restriction	Open
Rights Holder	Oxford University Press
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics

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Exploring the DNA mimicry of the Ocr protein of phage T7

Dissection of the DNA Mimicry of the Bacteriophage T7 Ocr Protein using Chemical Modification

Phage T7 DNA mimic protein Ocr is a potent inhibitor of BREX defence

Phage T7 DNA mimic protein Ocr is a potent inhibitor of BREX defence

Characterisation of the structure of ocr, the gene 0.3 protein of bacteriophage T7

Antirestriction activity of the monomeric and dimeric forms of T7 Ocr

Localization of low-melting regions in phage T7 DNA

Interaction of the ocr gene 0.3 protein of bacteriophage T7 with EcoKI restriction/modification enzyme

Specific fragmentation of T7 phage DNA at low-melting sites.

Exploring the DNA mimicry of the Ocr protein of phage T7

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Exploring the DNA mimicry of the Ocr protein of phage T7

Dissection of the DNA Mimicry of the Bacteriophage T7 Ocr Protein using Chemical Modification

Phage T7 DNA mimic protein Ocr is a potent inhibitor of BREX defence

Phage T7 DNA mimic protein Ocr is a potent inhibitor of BREX defence

Characterisation of the structure of ocr, the gene 0.3 protein of bacteriophage T7

Antirestriction activity of the monomeric and dimeric forms of T7 Ocr

Localization of low-melting regions in phage T7 DNA

Interaction of the ocr gene 0.3 protein of bacteriophage T7 with EcoKI restriction/modification enzyme

Specific fragmentation of T7 phage DNA at low-melting sites.

Exploring the DNA mimicry of the Ocr protein of phage T7