NDLI: Effects of the SpoVT Regulatory Protein on the Germination and Germination Protein Levels of Spores of Bacillus subtilis

Content Provider	PubMed Central
Author	Arturo, Ramirez-peralta Stewart, Kerry-ann V. Thomas, Stacy K. Barbara, Setlow Chen, Zhan Li, Yong-qing Peter, Setlow
Copyright Year	2012
Abstract	Bacillus subtilis isolates lacking the SpoVT protein, which regulates gene expression in developing forespores, gave spores that released their dipicolinic acid (DPA) via germinant receptor (GR)-dependent germination more rapidly than wild-type spores. Non-GR-dependent germination via dodecylamine was more rapid with spoVT spores, but germination via Ca-DPA was slower. The effects of a spoVT mutation on spore germination were seen with spores made in rich and poor media, and levels of SpoVT-LacZ were elevated 2-fold in poor-medium spores; however, elevated SpoVT levels were not the only cause of the slower GR-dependent germination of poor-medium spores. The spoVT spores had ≥5-fold higher GerA GR levels, ∼2-fold elevated GerB GR levels, wild-type levels of a GerK GR subunit and the GerD protein required for normal GR-dependent germination, ∼2.5-fold lower levels of the SpoVAD protein involved in DPA release in spore germination, and 30% lower levels of DNA protective α/β-type small, acid-soluble spore proteins. With one exception, the effects on protein levels in spoVT spores are consistent with the effects of SpoVT on forespore transcription. The spoVT spores were also more sensitive to UV radiation and outgrew slowly. While spoVT spores' elevated GR levels were consistent with their more rapid GR-dependent germination, detailed analysis of the results suggested that there is another gene product crucial for GR-dependent spore germination that is upregulated in the absence of SpoVT. Overall, these results indicate that SpoVT levels during spore formation have a major impact on the germination and the resistance of the resultant spores.
Related Links	http://dx.doi.org/10.1128/jb.00504-12
Ending Page	3425
Page Count	9
Starting Page	3417
File Format	PDF
ISSN	00219193
e-ISSN	10985530
Journal	Journal of Bacteriology
Issue Number	13
Volume Number	194
Language	English
Publisher	American Society for Microbiology
Publisher Date	2012-07-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Molecular Biology Microbiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Microbiology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Germination Protein Levels and Rates of Germination of Spores of Bacillus subtilis with Overexpressed or Deleted Genes Encoding Germination Proteins

Effects of Sporulation Conditions on the Germination and Germination Protein Levels of Bacillus subtilis Spores

Levels of Germination Proteins in Dormant and Superdormant Spores of Bacillus subtilis

Effects of a gerF (lgt) Mutation on the Germination of Spores of Bacillus subtilis

Effects of Overexpression of Nutrient Receptors on Germination of Spores of Bacillus subtilis

SpoVT: From Fine-Tuning Regulator in Bacillus subtilis to Essential Sporulation Protein in Bacillus cereus

Analysis of the Effects of a gerP Mutation on the Germination of Spores of Bacillus subtilis

Localization of the Germination Protein GerD to the Inner Membrane in Bacillus subtilis Spores▿

Role of GerD in Germination of Bacillus subtilis Spores▿

Effects of the SpoVT Regulatory Protein on the Germination and Germination Protein Levels of Spores of Bacillus subtilis

Similar Documents

Germination Protein Levels and Rates of Germination of Spores of Bacillus subtilis with Overexpressed or Deleted Genes Encoding Germination Proteins

Effects of Sporulation Conditions on the Germination and Germination Protein Levels of Bacillus subtilis Spores

Levels of Germination Proteins in Dormant and Superdormant Spores of Bacillus subtilis

Effects of a gerF (lgt) Mutation on the Germination of Spores of Bacillus subtilis

Effects of Overexpression of Nutrient Receptors on Germination of Spores of Bacillus subtilis

SpoVT: From Fine-Tuning Regulator in Bacillus subtilis to Essential Sporulation Protein in Bacillus cereus

Analysis of the Effects of a gerP Mutation on the Germination of Spores of Bacillus subtilis

Localization of the Germination Protein GerD to the Inner Membrane in Bacillus subtilis Spores▿

Role of GerD in Germination of Bacillus subtilis Spores▿

Effects of the SpoVT Regulatory Protein on the Germination and Germination Protein Levels of Spores of Bacillus subtilis