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“Smart” Drug Carriers: PEGylated TATp-Modified pH-Sensitive Liposomes
| Content Provider | PubMed Central |
|---|---|
| Author | Kale, Amit A. P., Torchilin Vladimir |
| Abstract | To engineer drug carriers capable of spontaneous accumulation in tumors and ischemic areas via the enhanced permeability and retention (EPR) effect and further penetration and drug delivery inside tumor or ischemic cells via the action of the cell-penetrating peptide (CPP), we have prepared liposomes simultaneously bearing on their surface CPP (TAT peptide, TATp) moieties and protective PEG chains. PEG chains were incorporated into the liposome membrane via the PEG-attached phosphatidylethanolamine (PE) residue with PEG and PE being conjugated with the lowered pH-degradable hydrazone bond (PEG-HZ-PE). Under normal conditions, liposome-grafted PEG “shielded” liposome-attached TATp moieties since the PEG spacer for TATp attachment (PEG1000) was shorter than protective PEG2000. PEGy-lated liposomes are expected to accumulate in targets via the EPR effect, but inside the “acidified” tumor or ischemic tissues lose their PEG coating due to the lowered pH-induced hydrolysis of HZ and penetrate inside cells via the now-exposed TATp moieties. This concept is shown here to work in cell cultures in vitro as well as in ischemic cardiac tissues in the Langendorff perfused rat heart model and in tumors in experimental mice in vivo. |
| Related Links | http://dx.doi.org/10.1080/08982100701525035 |
| Ending Page | 203 |
| Page Count | 7 |
| Starting Page | 197 |
| File Format | |
| ISSN | 08982104 |
| e-ISSN | 15322394 |
| Journal | Journal of liposome research |
| Issue Number | 3-4 |
| Volume Number | 17 |
| Language | English |
| Publisher Date | 2007-01-01 |
| Access Restriction | Open |
| Subject Keyword | Pharmacology Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Pharmaceutical Science |
