NDLI: Comparison of Scheffersomyces stipitis strains CBS 5773 and CBS 6054 with regard to their xylose metabolism: implications for xylose fermentation

Please wait, while we are loading the content...

Comparison of Scheffersomyces stipitis strains CBS 5773 and CBS 6054 with regard to their xylose metabolism: implications for xylose fermentation

Content Provider	PubMed Central
Author	Krahulec, Stefan Kratzer, Regina Longus, Karin Bernd, Nidetzky
Copyright Year	2012
Abstract	The various strains of Scheffersomyces stipitis (Pichia stipitis) differ substantially with respect to their ability to ferment xylose into ethanol. Two P. stipitis strains CBS 5773 and CBS 6054 have been most often used in literature but comparison of their performance in xylose fermentation under identical conditions has not been reported so far. Conversion of xylose (22 g/L) by each of these P. stipitis strain was analyzed under anaerobic and microaerobic conditions. Ethanol yields of ∼0.41 g/g were independent of strain and conditions used. Glycerol and acetate were formed in constant yields of 0.006 g/g and 0.02 g/g, respectively. Xylitol formation decreased from ∼0.08 g/g to ∼0.05 g/g upon switch from anaerobic to microaerobic conditions. Specific activities of enzymes of the two-step oxidoreductive xylose conversion pathway (xylose reductase and xylitol dehydrogenase) matched for both strains within limits of error. When xylose was offered at 76 g/L under microaerobic reaction conditions, ethanol yields were still high (0.37–0.39 g/g) for both strains even though the xylitol yields (0.12–0.13 g/g) were increased as compared to the conditions of low xylose concentration. P. stipitis strains CBS 5773 and CBS 6054 are therefore identical by the criteria selected and show useful performance during conversion of xylose into ethanol, irrespective of the supply of oxygen.
Related Links	http://dx.doi.org/10.1002/mbo3.5
Ending Page	70
Page Count	7
Starting Page	64
File Format	PDF
ISSN	20458827
e-ISSN	20458827
Journal	MicrobiologyOpen
Issue Number	1
Volume Number	1
Language	English
Publisher	Blackwell Publishing Inc
Publisher Date	2012-03-01
Access Restriction	Open
Rights Holder	Blackwell Publishing Inc
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Microbiology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Cloning novel sugar transporters from Scheffersomyces (Pichia) stipitis allowing d-xylose fermentation by recombinant Saccharomyces cerevisiae

Evaluation of ethanol production from corncob using Scheffersomyces (Pichia) stipitis CBS 6054 by volumetric scale-up.

Flux balance analysis predicts NADP phosphatase and NADH kinase are critical to balancing redox during xylose fermentation in Scheffersomyces stipitis

Conversion of $C_{6}$ and $C_{5}$ sugars in undetoxified wet exploded bagasse hydrolysates using Scheffersomyces (Pichia) stipitis CBS6054

Kinetic Modeling of Ethanol Production by Scheffersomyces stipitis from Xylose

Cloning and Characterization of Two Pyruvate Decarboxylase Genes from Pichia stipitis CBS 6054

Evaluation of Ethanol Production from Corncob Using Scheffersomyces (Pichia) stipitis CBS 6054 by Volumetric Scale-up

Effect of cell immobilization and pH on Scheffersomyces stipitis growth and fermentation capacity in rich and inhibitory media

Ethanol Reassimilation and Ethanol Tolerance in Pichia stipitis CBS 6054 as Studied by 13C Nuclear Magnetic Resonance Spectroscopy

Comparison of Scheffersomyces stipitis strains CBS 5773 and CBS 6054 with regard to their xylose metabolism: implications for xylose fermentation

Similar Documents

Cloning novel sugar transporters from Scheffersomyces (Pichia) stipitis allowing d-xylose fermentation by recombinant Saccharomyces cerevisiae

Evaluation of ethanol production from corncob using Scheffersomyces (Pichia) stipitis CBS 6054 by volumetric scale-up.

Flux balance analysis predicts NADP phosphatase and NADH kinase are critical to balancing redox during xylose fermentation in Scheffersomyces stipitis

Conversion of $C_{6}$ and $C_{5}$ sugars in undetoxified wet exploded bagasse hydrolysates using Scheffersomyces (Pichia) stipitis CBS6054

Kinetic Modeling of Ethanol Production by Scheffersomyces stipitis from Xylose

Cloning and Characterization of Two Pyruvate Decarboxylase Genes from Pichia stipitis CBS 6054

Evaluation of Ethanol Production from Corncob Using Scheffersomyces (Pichia) stipitis CBS 6054 by Volumetric Scale-up

Effect of cell immobilization and pH on Scheffersomyces stipitis growth and fermentation capacity in rich and inhibitory media

Ethanol Reassimilation and Ethanol Tolerance in Pichia stipitis CBS 6054 as Studied by 13C Nuclear Magnetic Resonance Spectroscopy

Comparison of Scheffersomyces stipitis strains CBS 5773 and CBS 6054 with regard to their xylose metabolism: implications for xylose fermentation