NDLI: A HIGH-THROUGHPUT FLUORESCENCE ACTIVATED NANOSCALE SUBCELLULAR SORTER WITH SINGLE-MOLECULE SENSITIVITY

Content Provider	PubMed Central
Author	Schiro, Perry G. Gadd, Jennifer C. Yen, Gloria S. Chiu, Daniel T.
Abstract	Recent single-cell and single-molecule studies have shown that a variety of subpopulations exist within biological systems, such as synaptic vesicles, that have previously been overlooked in common bulk studies. By isolating and enriching these various subpopulations, detailed analysis with a variety of analytical techniques can be done to further understand the role that various subpopulations play in cellular dynamics and how alterations to these subpopulations affect the overall function of the biological system. Previous sorters lack the sensitivity, sorting speed, and efficiency to isolate synaptic vesicles and other nanoscale systems. This paper describes the development of a fluorescence activated nanoscale subcellular sorter that can sort nearly 10 million objects per hour with single-molecule sensitivity. Utilizing a near-nanoscale channel system, we were able to achieve upwards of 91% recovery of desired objects with a 99.7% purity.
Related Links	http://dx.doi.org/10.1021/jp3019233
Starting Page	10490
File Format	PDF
ISSN	15205207
e-ISSN	15205207
Journal	The journal of physical chemistry. B
Issue Number	35
Volume Number	116
Language	English
Publisher Date	2012-09-06
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Surfaces, Coatings and Films Materials Chemistry Medicine Physical and Theoretical Chemistry

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A HIGH-THROUGHPUT FLUORESCENCE ACTIVATED NANOSCALE SUBCELLULAR SORTER WITH SINGLE-MOLECULE SENSITIVITY