NDLI: Sinorhizobium meliloti ExoR Is the Target of Periplasmic Proteolysis

Content Provider	PubMed Central
Author	Lu, Hai-yang Luo, Li Yang, Meng-hua Cheng, Hai-ping
Copyright Year	2012
Abstract	Sinorhizobium meliloti ExoR regulates the production of succinoglycan and flagella through the ExoS/ChvI two-component regulatory system. ExoR has been proposed to inhibit the ExoS sensor through direct interaction in the periplasm. To understand how ExoR suppression of ExoS is relieved, which is required for the expression of ExoS/ChvI-regulated symbiosis genes, we characterized wild-type ExoR and ExoR95 mutant proteins. In addition to the previously identified precursor and mature forms of ExoR (designated ExoRp and ExoRm, respectively), we detected a 20-kDa form of ExoR (designated ExoRc20) derived from the wild-type ExoR protein, but not from the ExoR95 mutant protein. ExoRc20 was isolated directly from S. meliloti periplasm to identify its N-terminal amino acids and the site of the proteolysis, which is highly conserved among ExoR homologs. ExoRc20 retains the C terminus of the wild-type ExoR. When expressed directly, ExoRc20 did not complement the exoR95 mutation, suggesting that ExoRc20 does not function directly in the ExoR-ExoS/ChvI regulatory pathway and that ExoRm is the functional form of ExoR. A single-amino-acid change (ExoRL81A) at the site of ExoR periplasmic proteolysis resulted in the reduction of the amount of ExoRm and the loss of the regulatory function of the ExoR protein. These findings suggest that ExoRm is a target of periplasmic proteolysis and that the amount of ExoRm could be reduced through effective proteolysis to relieve its suppression of ExoS.
Related Links	http://dx.doi.org/10.1128/jb.00313-12
Ending Page	4040
Page Count	12
Starting Page	4029
File Format	PDF
ISSN	00219193
e-ISSN	10985530
Journal	Journal of Bacteriology
Issue Number	15
Volume Number	194
Language	English
Publisher	American Society for Microbiology
Publisher Date	2012-08-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Molecular Biology Microbiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Microbiology

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