NDLI: Development of a Tightly Regulatable Copper-Mediated Gene Switch System in Dermatophytes

Content Provider	PubMed Central
Author	Iwata, Atsushi Mahdi, Alshahni Mohamed Nishiyama, Yayoi Makimura, Koichi Abe, Shigeru Yamada, Tsuyoshi
Copyright Year	2012
Abstract	Targeted gene deletion is now available for molecular genetic research of dermatophytes, and the physiological roles of several genes have been elucidated. However, this method cannot be applied to essential genes, which can be potential drug targets. To overcome this limitation, we have developed a conditional gene knockdown system using a copper-responsive promoter. The promoter sequence of the copper transporter gene CTR4 (P CTR4 ) and that of the copper efflux pump gene CRP1 (P CRP1 ) derived from Trichophyton rubrum were examined for their response to copper in Arthroderma vanbreuseghemii. P CTR4 was demonstrated to repress expression of a reporter gene in the presence of copper, while the activity of P CRP1 was induced by addition of copper. Importantly, P CTR4 regulated the gene expression more tightly. Furthermore, when P CTR4 was applied to regulate the expression of the endogenous genes ERG1 and TRP5, their conditional mutants exhibited decreased growth activity under the repressive conditions. These results suggest that the P CTR4 -based gene regulation system represents a powerful tool for identification and characterization of a broad range of genes, including essential genes, in dermatophytes.
Related Links	http://dx.doi.org/10.1128/aem.00464-12
Ending Page	5211
Page Count	8
Starting Page	5204
File Format	PDF
ISSN	00992240
e-ISSN	10985336
Journal	Applied and Environmental Microbiology
Issue Number	15
Volume Number	78
Language	English
Publisher	American Society for Microbiology
Publisher Date	2012-08-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Biotechnology Food Science Ecology Applied Microbiology and Biotechnology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Ecology Food Science Applied Microbiology and Biotechnology Biotechnology

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