NDLI: T cell responses to known allergen proteins are differently polarized and account for a variable fraction of total response to allergen extracts 1

Content Provider	PubMed Central
Author	Oseroff, Carla Sidney, John Vita, Randi Victoria, Tripple Mckinney, Denise M. Southwood, Scott Brodie, Tess M. Sallusto, Federica Grey, Howard Alam, Rafeul David, Broide Greenbaum, Jason A. Kolla, Ravi Peters, Bjoern Sette, Alessandro
Abstract	A panel of 133 allergens derived from 28 different sources, including fungi, trees, grasses, weeds and indoor allergens, was surveyed utilizing prediction of HLA class II binding peptides and ELISPOT assays with PBMC from allergic donors, resulting in the identification of 257 T cell epitopes. More than 90% of the epitopes were novel, and for 14 allergen sources were the first ever identified. The epitopes identified in the different allergen sources summed up to a variable fraction of the total extract response. In cases of allergens where the identified T cell epitopes accounted for a minor fraction of the extract response, fewer known protein sequences were available, suggesting that for “low epitope coverage” allergen sources, additional allergen proteins remain to be identified. IL-5 and IFN-γresponses were measured as prototype Th2 and Th1 responses, respectively. While in some cases (e.g., Orchard Grass, Alternaria, Cypress, and Russian Thistle) IL-5 production greatly exceeded IFN-γ, in others (e.g., Aspergillus, Penicillum, and Alder) the production of IFN-γ exceeded IL-5. Thus, different allergen sources are associated with variable polarization of the responding T cells. The present study represents the most comprehensive survey to date of human allergen derived T cell epitopes. These epitopes might be used to characterize T cell phenotype/T cell plasticity as a function of seasonality, or as a result of SIT treatment or varying disease severity (asthma or rhinitis).
Related Links	http://dx.doi.org/10.4049/jimmunol.1200850
Ending Page	1811
Page Count	12
Starting Page	1800
File Format	PDF
ISSN	00221767
e-ISSN	15506606
Journal	Journal of immunology (Baltimore, Md. : 1950)
Issue Number	4
Volume Number	189
Language	English
Publisher Date	2012-08-15
Access Restriction	Open
Subject Keyword	Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

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