NDLI: AvrPto-dependent Pto-interacting proteins and AvrPto-interacting proteins in tomato

Content Provider	PubMed Central
Author	Bogdanove, Adam J. Martin, Gregory B.
Copyright Year	2000
Abstract	The plant-intracellular interaction of the avirulence protein AvrPto of Pseudomonas syringae pathovar tomato, the agent of bacterial speck disease, and the corresponding tomato resistance protein Pto triggers responses leading to disease resistance. Pto, a serine/threonine protein kinase, also interacts with a putative downstream kinase, Pto-interactor 1, as well as with members of a family of transcription factors Pto-interactors 4, 5, and 6. These proteins are likely involved, respectively, in a phosphorylation cascade resulting in hypersensitive cell death, and in defense gene activation. The mechanism by which the interaction of AvrPto and Pto initiates defense response signaling is not known. To pursue the hypothesis that tertiary interactions are involved, we modified the yeast two-hybrid protein interaction trap and conducted a search for tomato proteins that interact with Pto only in the presence of AvrPto. Five classes of AvrPto-dependent Pto interactors were isolated, and their interaction specificity confirmed. Also, to shed light on a recently demonstrated virulence activity of AvrPto, we conducted a standard two-hybrid screen for tomato proteins in addition to Pto that interact with AvrPto: i.e., potential virulence targets or modifiers of AvrPto. By constructing an N-terminal rather than a C-terminal fusion of AvrPto to the LexA DNA binding domain, we were able to overcome autoactivation by AvrPto and identify four classes of specific AvrPto-interacting proteins.
Starting Page	8836
File Format	PDF
ISSN	10916490
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	16
Volume Number	97
Language	English
Publisher	The National Academy of Sciences
Publisher Date	2000-08-01
Access Restriction	Open
Rights Holder	The National Academy of Sciences
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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AvrPto-dependent Pto-interacting proteins and AvrPto-interacting proteins in tomato.

The T-loop Extension of the Tomato Protein Kinase AvrPto-dependent Pto-interacting Protein 3 (Adi3) Directs Nuclear Localization for Suppression of Plant Cell Death*

The cloned avirulence gene avrPto induces disease resistance in tomato cultivars containing the Pto resistance gene.

Pto Mutants Differentially Activate Prf-Dependent, avrPto-Independent Resistance and Gene-for-Gene Resistance1

Crystal Structure of the Complex between Pseudomonas Effector AvrPtoB and the Tomato Pto Kinase Reveals Both a Shared and a Unique Interface Compared with AvrPto-Pto[W]

Constitutively active Pto induces a Prf-dependent hypersensitive response in the absence of avrPto.

Expression of the Tomato Pto Gene in Tobacco Enhances Resistance to Pseudomonas syringae pv tabaci Expressing avrPto.

The T-loop Extension of the Tomato Protein Kinase AvrPto-dependent Pto-interacting Protein 3 (Adi3) Directs Nuclear Localization for Suppression of Plant Cell Death

The cloned avirulence gene avrPto induces disease resistance in tomato cultivars containing the Pto resistance gene.

AvrPto-dependent Pto-interacting proteins and AvrPto-interacting proteins in tomato

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AvrPto-dependent Pto-interacting proteins and AvrPto-interacting proteins in tomato.

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