NDLI: Inhibitory Effects of Arginine on the Aggregation of Bovine Insulin

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Inhibitory Effects of Arginine on the Aggregation of Bovine Insulin

Content Provider	PubMed Central
Author	Varughese, Michael M. Newman, Jay
Copyright Year	2012
Abstract	Static and dynamic light scattering were used to investigate the effects of L-arginine, commonly used to inhibit protein aggregation, on the initial aggregation kinetics of solutions of bovine insulin in 20% acetic acid and 0.1 M NaCl as a model system for amyloidosis. Measurements were made as a function of insulin concentration (0.5–2.0 mM), quench temperature (60–85°C), and arginine concentration (10–500 mM). Aggregation kinetics under all conditions had a lag phase, whose duration decreased with increasing temperature and with increasing insulin concentration but which increased by up to a factor of 8 with increasing added arginine. Further, the initial growth rate after the lag phase also slowed by up to a factor of about 20 in the presence of increasing concentrations of arginine. From the temperature dependence of the lag phase duration, we find that the nucleation activation energy doubles from 17 ± 5 to 36 ± 3 kcal/mol in the presence of 500 mM arginine.
Related Links	http://dx.doi.org/10.1155/2012/434289
Starting Page	434289
File Format	PDF
ISSN	16878000
e-ISSN	16878019
Journal	Journal of Biophysics
Volume Number	2012
Language	English
Publisher	Hindawi Publishing Corporation
Publisher Date	2012-01-01
Access Restriction	Open
Rights Holder	Hindawi Publishing Corporation
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Biophysics Biomedical Engineering

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