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| Content Provider | PubMed Central |
|---|---|
| Author | Villegas-rosales, Paula M. Alfonso, Méndez-tenorio Elizabeth, Ortega-soto Barrón, Blanca L. |
| Copyright Year | 2012 |
| Abstract | Dengue virus (DENV 1-4) represents the major emerging arthropod-borne viral infection in the world. Currently, there is neitheran available vaccine nor a specific treatment. Hence, there is a need of antiviral drugs for these viral infections; we describe theprediction of short interfering RNA (siRNA) as potential therapeutic agents against the four DENV serotypes. Our strategy was tocarry out a series of multiple alignments using ClustalX program to find conserved sequences among the four DENV serotypegenomes to obtain a consensus sequence for siRNAs design. A highly conserved sequence among the four DENV serotypes,located in the encoding sequence for NS4B and NS5 proteins was found. A total of 2,893 complete DENV genomes weredownloaded from the NCBI, and after a depuration procedure to identify identical sequences, 220 complete DENV genomes wereleft. They were edited to select the NS4B and NS5 sequences, which were aligned to obtain a consensus sequence. Three differentservers were used for siRNA design, and the resulting siRNAs were aligned to identify the most prevalent sequences. ThreesiRNAs were chosen, one targeted the genome region that codifies for NS4B protein and the other two; the region for NS5 protein.Predicted secondary structure for DENV genomes was used to demonstrate that the siRNAs were able to target the viral genomeforming double stranded structures, necessary to activate the RNA silencing machinery. |
| Related Links | http://dx.doi.org/10.6026/97320630008519 |
| Ending Page | 522 |
| Page Count | 4 |
| Starting Page | 519 |
| File Format | |
| ISSN | 09738894 |
| e-ISSN | 09732063 |
| Journal | Bioinformation |
| Issue Number | 11 |
| Volume Number | 8 |
| Language | English |
| Publisher | Biomedical Informatics |
| Publisher Date | 2012-06-01 |
| Access Restriction | Open |
| Rights Holder | Biomedical Informatics |
| Subject Keyword | Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
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