NDLI: Quantitative Evaluation of DNA Hypermethylation in Malignant and Benign Breast Tissue and Fluids

Content Provider	PubMed Central
Author	Zhu, Weizhu Qin, Wenyi Hewett, John E. Sauter, Edward R.
Abstract	The assessment of DNA had demonstrated altered methylation in malignant compared to benign breast tissue.The purpose of our study was to 1) confirm the predictive ability of methylation assessment in breast tissue, and 2) use the genes found to be cancer predictive in tissue to evaluate the diagnostic potential of hypermethylation assessment in nipple aspirate fluid (NAF) and mammary ductoscopic (MD) samples. Quantitative methylation specific (qMS)-PCR was conducted on three specimen sets: 44 malignant (CA) and 34 normal (NL) tissue specimens, 18 matched CA, adjacent normal (ANL) tissue and NAF specimens, and 119 MD specimens. Training and validation tissue sets were analyzed to determine the optimal group of cancer predictive genes for NAF and MD analysis. NAF and MD cytologic review were also performed. Methylation of CCND-2, p16, RAR-β and RASSF-1a was significantly more prevalent in tumor than in normal tissue specimens. Receiver operating characteristic curve analysis demonstrated an area under the curve of 0.96. For the 18 matched CA, ANL and NAF specimens, the four predictive genes identified in cancer tissue contained increased methylation in CA vs. ANL tissue; NAF samples had higher methylation than ANL specimens. Methylation frequency was higher in MD specimens from breasts with cancer than benign samples for p16 and RASSF-1a. In summary, 1) routine quantitative DNA methylation assessment in NAF and MD samples is possible, and 2) genes hypermethylated in malignant breast tissue are also altered in matched NAF and in MD samples, and may be useful to assist in early breast cancer detection.
Related Links	http://dx.doi.org/10.1002/ijc.24728
Ending Page	482
Page Count	9
Starting Page	474
File Format	PDF
ISSN	00207136
e-ISSN	10970215
Journal	International Journal of Cancer. Journal International du Cancer
Issue Number	2
Volume Number	126
Language	English
Publisher Date	2010-01-15
Access Restriction	Open
Subject Keyword	Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cancer Research Oncology

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