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Mutations in the pqe-1 Gene Enhance Transgene Expression in Caenorhabditis elegans

Content Provider	PubMed Central
Author	Yamada, Koji Tsuchiya, Jun-ichi Iino, Yuichi
Copyright Year	2012
Abstract	Although various genetic tools have been developed and used as transgenes, the expression of the transgenes often is hampered by negative regulators. Disrupting such negative regulators of gene expression is potentially a way to overcome the common problem of low expression of transgenes. To find such regulators whose mutations enhance transgene expression in Caenorhabditis elegans, we took advantage of a newly developed reporter transgene, lin-11pAΔ::venus. This transgene induces expression of a fluorescent protein, Venus, in specific neurons including AIZ, where the expression was stochastic. The frequency of reporter expression in AIZ seemed to be correlated with the strength of transgene expression. By using this system, in which a moderate increase of expression was converted to all-or-none expression states, we describe here a forward genetic screen for mutations that enhance the expression of transgenes. Through the screen, we found that mutations in the pqe-1 gene, which encodes a Q/P-rich nuclear protein with an exonuclease domain, increase the chance of reporter expression in AIZ. The fluorescence intensity in RIC, in which all lin-11pAΔ::venus animals show reporter expression, was increased in pqe-1 mutants, suggesting that pqe-1 reduces the expression level of the transgene. Expression of transgenes with other promoters, 3′UTR, or reporter genes was also enhanced by the pqe-1 mutation, suggesting that the effect was not specific to a particular type of transgenes, whereas the effect did not seem to extend to endogenous genes. We propose that pqe-1 mutants can be used to increase the expression of various useful transgenes.
Related Links	http://dx.doi.org/10.1534/g3.112.002832
Ending Page	751
Page Count	11
Starting Page	741
File Format	PDF
ISSN	21601836
e-ISSN	21601836
Journal	G3: Genes\|Genomes\|Genetics
Issue Number	7
Volume Number	2
Language	English
Publisher	Genetics Society of America
Publisher Date	2012-07-01
Access Restriction	Open
Rights Holder	Genetics Society of America
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics Molecular Biology Genetics (clinical)

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