NDLI: Biochemical Characterization of APPL Endosomes: The Role of Annexin A2 in APPL Membrane Recruitment

Content Provider	PubMed Central
Author	Urbanska, Anna Sadowski, Lukasz Yannis, Kalaidzidis Miaczynska, Marta
Copyright Year	2011
Abstract	APPL endosomes are a recently identified subpopulation of early endosomes characterized by the presence of two homologous Rab5 effector proteins APPL1 and APPL2. They exhibit only limited colocalization with EEA1, another Rab5 effector and a marker of the canonical early endosomes. Although APPL endosomes appear to play important roles in cargo trafficking and signal transduction, their protein composition and biochemical properties remain largely unknown. Here we employed membrane fractionation methods to characterize APPL endosomes biochemically. We demonstrate that they represent heterogeneous membrane structures which can be discriminated from the canonical EEA1-positive early endosomes by their partly different physical properties and a distinct migration pattern in the continuous density gradients. In search for other potential markers of APPL endosomes we identified Annexin A2 as an interacting partner of both APPL1 and APPL2. Annexin A2 is a Ca2+ and phosphatidylinositol 4,5-bisphosphate binding protein, previously implicated in several endocytic steps. We show that Annexin A2 co-fractionates and colocalizes with APPL endosomes. Moreover, silencing of its expression causes solubilization of APPL2 from endosomes. Although Annexin A2 is not an exclusive marker of APPL endosomes, our data suggest that it has an important function in membrane recruitment of APPL proteins, acting in parallel to Rab5.
Related Links	http://dx.doi.org/10.1111/j.1600-0854.2011.01226.x
Starting Page	1227
File Format	PDF
ISSN	16000854
e-ISSN	16000854
Journal	Traffic (Copenhagen, Denmark)
Issue Number	9
Volume Number	12
Language	English
Publisher	Blackwell Publishing Ltd
Publisher Date	2011-09-01
Access Restriction	Open
Rights Holder	Blackwell Publishing Ltd
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Genetics Structural Biology Molecular Biology Biochemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Lipid Segregation and Membrane Budding Induced by the Peripheral Membrane Binding Protein Annexin A2*

The p11/S100A10 Light Chain of Annexin A2 Is Dispensable for Annexin A2 Association to Endosomes and Functions in Endosomal Transport

A Role for Phospholipase A2 Activity in Membrane Tubule Formation and TGN Trafficking

Dynamic reciprocity: the role of annexin A2 in tissue integrity

The S100A10-annexin A2 complex provides a novel asymmetric platform for membrane repair.

The S100A10-Annexin A2 Complex Provides a Novel Asymmetric Platform for Membrane Repair*

Lipid segregation and membrane budding induced by the peripheral membrane binding protein annexin A2.

Annexin A2 Binding to Endosomes and Functions in Endosomal Transport Are Regulated by Tyrosine 23 Phosphorylation

Characterization of phospholipase A2 in monocytic cell lines. Functional and biochemical aspects of membrane association.

Biochemical Characterization of APPL Endosomes: The Role of Annexin A2 in APPL Membrane Recruitment

Similar Documents

Lipid Segregation and Membrane Budding Induced by the Peripheral Membrane Binding Protein Annexin A2*

The p11/S100A10 Light Chain of Annexin A2 Is Dispensable for Annexin A2 Association to Endosomes and Functions in Endosomal Transport

A Role for Phospholipase A2 Activity in Membrane Tubule Formation and TGN Trafficking

Dynamic reciprocity: the role of annexin A2 in tissue integrity

The S100A10-annexin A2 complex provides a novel asymmetric platform for membrane repair.

The S100A10-Annexin A2 Complex Provides a Novel Asymmetric Platform for Membrane Repair*

Lipid segregation and membrane budding induced by the peripheral membrane binding protein annexin A2.

Annexin A2 Binding to Endosomes and Functions in Endosomal Transport Are Regulated by Tyrosine 23 Phosphorylation

Characterization of phospholipase A2 in monocytic cell lines. Functional and biochemical aspects of membrane association.

Biochemical Characterization of APPL Endosomes: The Role of Annexin A2 in APPL Membrane Recruitment