NDLI: Multiple Pathways Suppress Telomere Addition to DNA Breaks in the Drosophila Germline

Content Provider	PubMed Central
Author	Michelle, Beaucher Zheng, Xiao-feng Amariei, Flavia Rong, Yikang S.
Copyright Year	2012
Abstract	Telomeres protect chromosome ends from being repaired as double-strand breaks (DSBs). Just as DSB repair is suppressed at telomeres, de novo telomere addition is suppressed at the site of DSBs. To identify factors responsible for this suppression, we developed an assay to monitor de novo telomere formation in Drosophila, an organism in which telomeres can be established on chromosome ends with essentially any sequence. Germline expression of the I-SceI endonuclease resulted in precise telomere formation at its cut site with high efficiency. Using this assay, we quantified the frequency of telomere formation in different genetic backgrounds with known or possible defects in DNA damage repair. We showed that disruption of DSB repair factors (Rad51 or DNA ligase IV) or DSB sensing factors (ATRIP or MDC1) resulted in more efficient telomere formation. Interestingly, partial disruption of factors that normally regulate telomere protection (ATM or NBS) also led to higher frequencies of telomere formation, suggesting that these proteins have opposing roles in telomere maintenance vs. establishment. In the ku70 mutant background, telomere establishment was preceded by excessive degradation of DSB ends, which were stabilized upon telomere formation. Most strikingly, the removal of ATRIP caused a dramatic increase in telomeric retrotransposon attachment to broken ends. Our study identifies several pathways thatsuppress telomere addition at DSBs, paving the way for future mechanistic studies.
Related Links	http://dx.doi.org/10.1534/genetics.112.138818
Starting Page	407
File Format	PDF
ISSN	19432631
e-ISSN	19432631
Journal	Genetics
Issue Number	2
Volume Number	191
Language	English
Publisher	Genetics Society of America
Publisher Date	2012-06-01
Access Restriction	Open
Rights Holder	Genetics Society of America
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Healing of Euchromatic Chromosome Breaks by Efficient de novo Telomere Addition in Drosophila melanogaster

Telomere Loss Provokes Multiple Pathways to Apoptosis and Produces Genomic Instability in Drosophila melanogaster

DNA damage signalling prevents deleterious telomere addition at DNA breaks

When the Ends Justify the Means: Regulation of Telomere Addition at Double-Strand Breaks in Yeast

Efficient repair of DNA breaks in Drosophila: evidence for single-strand annealing and competition with other repair pathways.

Telomere elongation is under the control of the RNAi-based mechanism in the Drosophila germline

DNA breaks are masked by multiple Rap1 binding in yeast: implications for telomere capping and telomerase regulation

Regulation of telomere addition at DNA double-strand breaks

Conserved DNA sequences adjacent to chromosome fragmentation and telomere addition sites in Euplotes crassus.

Multiple Pathways Suppress Telomere Addition to DNA Breaks in the Drosophila Germline

Similar Documents

Healing of Euchromatic Chromosome Breaks by Efficient de novo Telomere Addition in Drosophila melanogaster

Telomere Loss Provokes Multiple Pathways to Apoptosis and Produces Genomic Instability in Drosophila melanogaster

DNA damage signalling prevents deleterious telomere addition at DNA breaks

When the Ends Justify the Means: Regulation of Telomere Addition at Double-Strand Breaks in Yeast

Efficient repair of DNA breaks in Drosophila: evidence for single-strand annealing and competition with other repair pathways.

Telomere elongation is under the control of the RNAi-based mechanism in the Drosophila germline

DNA breaks are masked by multiple Rap1 binding in yeast: implications for telomere capping and telomerase regulation

Regulation of telomere addition at DNA double-strand breaks

Conserved DNA sequences adjacent to chromosome fragmentation and telomere addition sites in Euplotes crassus.

Multiple Pathways Suppress Telomere Addition to DNA Breaks in the Drosophila Germline