NDLI: EXAFS and NRVS Reveal a Conformational Distortion of the FeMo-cofactor in the MoFe Nitrogenase Propargyl Alcohol Complex

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EXAFS and NRVS Reveal a Conformational Distortion of the FeMo-cofactor in the MoFe Nitrogenase Propargyl Alcohol Complex

Content Provider	PubMed Central
Author	George, Simon J. Barney, Brett M. Mitra, Devrani Igarashi, Robert Y. Guo, Yisong Dean, Dennis R. Cramer, Stephen P. Seefeldt, Lance C.
Copyright Year	2012
Abstract	We have used EXAFS and NRVS spectroscopies to examine the structural changes in the FeMo-cofactor active site of the α-70Ala variant of Azotobacter vinelandii nitrogenase on binding and reduction of propargyl alcohol (PA). The Mo K-edge near-edge and EXAFS spectra are very similar in the presence and absence of PA, suggesting PA does not bind at Mo. By contrast, Fe EXAFS spectra show a clear and reproducible change in the long Fe-Fe interaction at ~3.7 Å on PA binding with the apparent appearance of a new Fe-Fe interaction at 3.99 Å. An analogous change in the long Mo-Fe 5.1 Å interaction is not seen. The NRVS spectra exclude the possibility of large-scale structural change of the FeMo-cofactor involving breaking the μ2 Fe-S-Fe bonds of the Fe6S9X core. The simplest chemically consistent structural change is that the bound form of PA is coordinated at Fe atoms (Fe6 or Fe7) adjacent to the Mo terminus, with a concomitant movement of the Fe away from the central atom X and along the Fe-X bond by about 0.35 Å. This study comprises the first experimental evidence of the conformational changes of the FeMo-cofactor active site on binding a substrate or product.
Related Links	http://dx.doi.org/10.1016/j.jinorgbio.2012.02.004
Ending Page	92
Page Count	8
Starting Page	85
File Format	PDF
ISSN	01620134
e-ISSN	18733344
Journal	Journal of Inorganic Biochemistry
Volume Number	112
Language	English
Publisher Date	2012-07-01
Access Restriction	Open
Subject Keyword	Inorganic Chemistry Biochemistry Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Biochemistry Inorganic Chemistry

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