NDLI: Synthesis and hybridization of a series of biotinylated oligonucleotides.

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Specific binding of cruciform DNA structures by a protein from human extracts.

The distribution of tightly bound proteins along the DNA chain reflects the type of cell differentiation.

Functional analysis of the p10 gene 5' leader sequence of the Autographa californica nuclear polyhedrosis virus.

Diethyl pyrocarbonate can detect a modified DNA structure induced by the binding of quinoxaline antibiotics.

Ligation of oligonucleotides to nucleic acids or proteins via disulfide bonds.

An ACCC-containing protein-binding sequence in the neighbourhood of the decanucleotide recognition site of the immunoglobulin gene promoter.

The cloning, purification and characterization of the Eco RV modification methylase.

Site-directed modification of DNA duplexes by chemical ligation.

Transcriptional terminators in the caa-cal operon and cai gene.

cDNA cloning of human hnRNP protein A1 reveals the existence of multiple mRNA isoforms.

Replication-induced stimulation of the major late promoter of adenovirus is correlated to the binding of a factor to sequences in the first intron.

Physical and gene organization of mitochondrial DNA in fertile and male sterile sunflower. CMS-associated alterations in structure and transcription of the atpA gene.

Upstream sequences required for transcription of the TFIIIA gene in Xenopus oocytes.

The Ds1 transposable element acts as an intron in the mutant allele Adh1-Fm335 and is spliced from the message.

The integration host factor of Escherichia coli binds to multiple sites at plasmid R6K gamma origin and is essential for replication.

Characterization of products derived from self-splicing of intron aI5 alpha which is located in the mitochondrial COX I gene of Saccharomyces cerevisiae.

Mapping genomic organization by field inversion and two-dimensional gel electrophoresis: application to the murine T-cell receptor gamma gene family.

Adenosine-guanosine preferential photocleavage of DNA by azido-benzoyl- and diazocyclopenta-dienylcarbonyloxy derivatives of 9-aminoacridine.

Characterization of repetitive sequence families in mouse heart small polydisperse circular DNAs: age-related studies.

DNA unwinding and inhibition of T4 DNA ligase by anthracyclines.

Reduced DNA topoisomerase II activity in ataxia-telangiectasia cells.

Bends in SV40 DNA: use of mutagenesis to identify the critical bases involved.

Direct visualization of single copy genes on banded metaphase chromosomes by nonisotopic in situ hybridization.

Cell-specific expression of transfected brain identifier repetitive DNAs.

Z DNA and loop structures by immunoelectronmicroscopy of supercoiled pRW751, a plasmid containing left-handed helices.

EcoRII can be activated to cleave refractory DNA recognition sites.

Autolytic processing of a phosphorothioate diester bond.

The effect on the function of the transcriptional activator NtrC from Klebsiella pneumoniae of mutations in the DNA-recognition helix.

Close relationship between non-viral retroposons in Drosophila melanogaster.

Analysis of the entire nucleotide sequence of the cryptic plasmid of Chlamydia trachomatis serovar L1. Evidence for involvement in DNA replication.

Accuracy of intrachromosomal gene conversion in mouse cells.

Synthesis and hybridization of a series of biotinylated oligonucleotides.

3' end of the malEFG operon in E.coli: localization of the transcription termination site.

Codon preference reflects mistranslational constraints: a proposal.

Histone genes of Volvox carteri: DNA sequence and organization of two H3-H4 gene loci.

Heterologous 5' flanking regions do not support in vitro template activity of a Drosophila melanogaster tRNA(Val3b) gene.

Nucleotide sequences of cytosolic 5S ribosomal RNAs from two gymnosperms, Gnetum gnemon and Ephedra kokanica.

Nucleotide sequence of the 18S rDNA from the microalga Nanochlorum eucaryotum.

DNA damage produced by ethidium bromide staining and exposure to ultraviolet light.

The complete nucleotide sequence of the arcaebacterial plasmid pHSB from Halobacterium, strain SB3.

Utility of firefly luciferase as a reporter gene for promoter activity in transgenic mice.

Effect of cytosine methylation on the cleavage of oligonucleotide duplexes with restriction endonucleases HpaII and MspI.

Improved hybridization conditions for DNA 'fingerprints' probed with M13.

Inosine incorporation in GC rich RNA probes increases hybridization sequence specificity.

pW-ATG-lac, P-element vectors for lacZ transcriptional gene fusions in Drosophila.

Magnesium-dependence of in vitro translation programmed by gene-specific mRNAs.

Detection of HIV-1 RNA sequences by in vitro DNA amplification.

Probing the human genome with minisatellite-like sequences from the human coagulation factor VII gene.

The nucleotide sequence of a rat vascular smooth muscle alpha-actin cDNA.

Bacillus sphaericus strain 2297: nucleotide sequence of 41.9 kDa toxin gene.

Sequence of integrated S-1 homologous DNA in the normal maize mitochondrial genome.

DpnI assay for DNA replication in animal cells: enzyme-resistant material can result from factors not related to replication.

Separation of episomal Epstein-Barr virus from Burkitt's lymphoma host cell DNA in pulse field gels.

Nucleotide sequence of the gene encoding the monocyte differentiation antigen, CD14.

Nucleotide sequence of the gene coding for the large subunit of ribonucleotide reductase of Escherichia coli. Correction.

Promoter of a somatic histone H2B gene of the sea urchin.

Nucleotide sequences of two soybean U1 snRNA genes.

S2 plasmid from cms-S-maize mitochondria potentially encodes a specific RNA polymerase.

RspX I: a new restriction endonuclease with a recognition sequence of 5'T/CATGA3'.

Simple and efficient method for cloning of large DNA fragments with identical ends into plasmid vectors.

Possible evolutionary relationship between poliovirus RNA-linked protein VPg and PRD1 DNA-terminal protein.

Complete sequence of a cDNA clone of the hemagglutinin gene of influenza A/Chicken/Scotland/59 (H5N1) virus: comparison with contemporary North American and European strains.

Human TaqI RFLP recognized by neurofilament gene probes.

RFLP for TaqI at the human neurofilament (NF-L) gene locus.

Isolation and mapping of a polymorphic DNA sequence (pTB10.163) on chromosome 10 [D10S22]

Isolation and mapping of a polymorphic DNA sequence (pYNZ32) on chromosome 4p [D4S125]

Isolation and mapping of a polymorphic DNA sequence (pTB10.171) on chromosome 10 [D10S19]

Isolation and mapping of a polymorphic DNA sequence (pMCT108.2) on chromosome 18 [D18S24]

Isolation and mapping of a polymorphic DNA sequence (pHHH163) on chromosome 18 [D18S21]

Isolation and mapping of a polymorphic DNA sequence (pCMM17.4) on chromosome 10 [D10S23]

Isolation and mapping of a polymorphic DNA sequence (pJCZ67) on chromosome 7 [D7S396]

Isolation and mapping of a polymorphic DNA sequence (pCMI37) on chromosome 7 [D7S368]

RFLP of the human c-fes proto-oncogene.

Cloning and characterization of a human ribosomal protein gene with enhanced expression in fetal and neoplastic cells

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Synthesis and hybridization of a series of biotinylated oligonucleotides.

Content Provider	PubMed Central
Author	Cook, A. F. Vuocolo, E. Brakel, C. L.
Abstract	A series of oligonucleotides containing biotin-11-dUMP at various positions were synthesized and compared in quantitative, colorimetric hybridization-detection studies. A deoxyuridine phosphoramidite containing a protected allylamino sidearm was synthesized and used in standard, automated synthesis cycles to prepare oligonucleotides with allylamino residues at various positions within a standard 17-base sequence. Biotin substituents were subsequently attached to the allylamino sidearms by reaction with N-biotinyl-6-aminocaproic acid N-hydroxysuccinimide ester. These oligomers were hybridized to target DNA immobilized on microtiter wells (ELISA plates), and were detected with a streptavidin-biotinylated horseradish peroxidase complex using hydrogen peroxide as substrate and o-phenylenediamine as chromogen. We found that the sensitivity of detection of target DNA by biotin-labeled oligonucleotide probes was strongly dependent upon the position of the biotin label. Oligonucleotides containing biotin labels near or off the ends of the hybridizing sequence were more effective probes than oligonucleotides containing internal biotin labels. An additive effect of increasing numbers of biotin-dUMP residues was found for some labeling configurations.
Starting Page	4077
File Format	PDF
ISSN	13624962
e-ISSN	13624962
Journal	Nucleic Acids Research
Issue Number	9
Volume Number	16
Language	English
Publisher Date	1988-05-11
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics

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