NDLI: Analysis of the cell surface layer ultrastructure of the oral pathogen Tannerella forsythia

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Analysis of the cell surface layer ultrastructure of the oral pathogen Tannerella forsythia

Content Provider	PubMed Central
Author	Gerhard, Sekot Posch, Gerald Oh, Yoo Jin Sonja, Zayni Mayer, Harald F. Dietmar, Pum Messner, Paul Peter, Hinterdorfer Schäffer, Christina
Abstract	The Gram-negative oral pathogen Tannerella forsythia is decorated with a 2D crystalline surface (S-) layer, with two different S-layer glycoprotein species being present. Prompted by the predicted virulence potential of the S-layer, this study focused on the analysis of the arrangement of the individual S-layer glycoproteins by a combination of microscopic, genetic, and biochemical analyses. The two S-layer genes are transcribed into mRNA and expressed into protein in equal amounts. The S-layer was investigated on intact bacterial cells by transmission electron microscopy, by immune fluorescence microscopy, and by atomic force microscopy. The analyses of wild-type cells revealed a distinct square S-layer lattice with an overall lattice constant of 10.1 ± 0.7 nm. In contrast, a blurred lattice with a lattice constant of 9.0 nm was found on S-layer single-mutant cells. This together with in vitro self-assembly studies using purified (glyco)protein species indicated their increased structural flexibility after self-assembly and/or impaired self-assembly capability. In conjunction with TEM analyses of thin-sectioned cells, this study demonstrates the unusual case that two S-layer glycoproteins are co-assembled into a single S-layer. Additionally, flagella and pilus-like structures were observed on T. forsythia cells, which might impact the pathogenicity of this bacterium.
Related Links	http://dx.doi.org/10.1007/s00203-012-0792-3
Ending Page	539
Page Count	15
Starting Page	525
File Format	PDF
ISSN	03028933
e-ISSN	1432072X
Journal	Archives of Microbiology
Issue Number	6
Volume Number	194
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-06-01
Access Restriction	Open
Rights Holder	Springer-Verlag
Subject Keyword	Genetics Biochemistry Molecular Biology Microbiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Biochemistry Molecular Biology Microbiology

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Analysis of the cell surface layer ultrastructure of the oral pathogen Tannerella forsythia

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