Loading...
Please wait, while we are loading the content...
Similar Documents
Zinc induces caspase-dependent mitochondrial pathway of the programmed cell death in haemocytes of Drosophila melanogaster
| Content Provider | PubMed Central |
|---|---|
| Author | Filipiak, Marta Tylko, Grzegorz Pyza, Elzbieta |
| Abstract | Zinc is an essential trace element in cells. However, its high level in cytoplasm promotes activation of stress signaling pathways and may lead to cell death. In the present study we used Drosophila melanogaster blood cells (haemocytes), obtained from the third instar larvae, to study the effects of high concentrations of Zn2+ on programmed cell death (PCD). We analyzed the activity of caspases, the level of caspase inhibitor protein DIAP1 and metallothioneins, as well as calcium concentrations and activity of mitochondria in haemocytes exposed to 0.35 and 1.7 mM concentrations of Zn. The obtained results showed that rapid increase of [Zn2+] i in the cytoplasm up-regulates metallothionein MtnB but not MtnA gene expression in cells treated with Zn2+ in both concentrations. Excess of Zn2+ also induced activation of the initiator caspase Dronc, associated with the mitochondrial pathway of PCD, and the effector caspase DrICE. In turn, the activity of receptor-regulated Dredd caspase was not changed. The level of DIAP1 decreased significantly in haemocytes in the presence of high Zn2+ concentration in comparison to untreated cells. Moreover, mitochondrial membrane potential was significantly decreased after exposure to Zn ions. These results indicate that high concentration of Zn2+ in the cytoplasm of haemocytes induces PCD via a mitochondrial pathway and that caspases play a pivotal role in this process. |
| Related Links | http://dx.doi.org/10.1007/s10534-012-9530-1 |
| Ending Page | 516 |
| Page Count | 10 |
| Starting Page | 507 |
| File Format | |
| ISSN | 09660844 |
| e-ISSN | 15728773 |
| Journal | Biometals |
| Issue Number | 3 |
| Volume Number | 25 |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2012-06-01 |
| Access Restriction | Open |
| Rights Holder | Springer Netherlands |
| Subject Keyword | Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) Metals and Alloys Biomaterials Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Metals and Alloys Biochemistry, Genetics and Molecular Biology Biomaterials Agricultural and Biological Sciences |
