NDLI: Long-term nose-only cigarette smoke exposure induces emphysema and mild skeletal muscle dysfunction in mice

Please wait, while we are loading the content...

An enhancer from the 8q24 prostate cancer risk region is sufficient to direct reporter gene expression to a subset of prostate stem-like epithelial cells in transgenic mice

ROS constitute a convergence nexus in the development of IGF1 resistance and impaired wound healing in a rat model of type 2 diabetes

neb: a zebrafish model of nemaline myopathy due to nebulin mutation

Generation of primary tumors with Flp recombinase in FRT-flanked p53 mice

Loss of PKBβ/Akt2 predisposes mice to ovarian cyst formation and increases the severity of polycystic ovary formation in vivo

Year: 2012, Volume: 5, Issue: 2

Year: 2012, Volume: 5, Issue: 1

Year: 2011, Volume: 4

Year: 2010, Volume: 3

Year: 2009, Volume: 2

Year: 2008, Volume: 1

Long-term nose-only cigarette smoke exposure induces emphysema and mild skeletal muscle dysfunction in mice

Content Provider	PubMed Central
Author	Rinaldi, Manuela Maes, Karen Vleeschauwer, Stéphanie De Thomas, Debby Verbeken, Erik K. Decramer, Marc Janssens, Wim Gayan-ramirez, Ghislaine N.
Copyright Year	2012
Abstract	Mouse models of chronic obstructive pulmonary disease (COPD) focus on airway inflammation and lung histology, but their use has been hampered by the lack of pulmonary function data in their assessment. Systemic effects such as muscle dysfunction are also poorly modeled in emphysematous mice. We aimed to develop a cigarette-smoke-induced emphysema mouse model in which serial lung function and muscular dysfunction could be assessed, allowing the disease to be monitored more appropriately. C57Bl6 mice were nose-only exposed to cigarette smoke or filtered air for 3–6 months. Lung function tests were repeated in the same mice after 3 and 6 months of cigarette smoke or air exposure and compared with lung histological changes. Contractile properties of skeletal muscles and muscle histology were also determined at similar time points in separate groups of mice. Serial lung function measurements documented hyperinflation after 3 and 6 months of cigarette smoke exposure, with a significant 31–37% increase in total lung capacity (TLC) and a significant 26–35% increase in compliance (Cchord) when compared with animals exposed to filtered air only (P<0.001 after 3 and after 6 months). These functional changes preceded the changes in mean linear intercept, which became only significant after 6 months of cigarette smoke exposure and which correlated very well with TLC (r=0.74, P=0.004) and Cchord (r=0.79, P=0.001). After 6 months of cigarette smoke exposure, a significant fiber-type shift from IIa to IIx/b was also observed in the soleus muscle (P<0.05), whereas a 20% reduction of force was present at high stimulation frequencies (80 Hz; P=0.09). The extensor digitorum longus (EDL) muscle was not affected by cigarette smoke exposure. These serial pulmonary function variables are sensitive outcomes to detect emphysema progression in a nose-only cigarette-smoke-exposed animal model of COPD. In this model, muscular changes became apparent only after 6 months, particularly in muscles with a mixed fiber-type composition.
Related Links	http://dx.doi.org/10.1242/dmm.008508
Ending Page	341
Page Count	9
Starting Page	333
File Format	PDF
ISSN	17548403
e-ISSN	17548411
Journal	Disease Models & Mechanisms
Issue Number	3
Volume Number	5
Language	English
Publisher	The Company of Biologists Limited
Publisher Date	2012-05-01
Access Restriction	Open
Rights Holder	The Company of Biologists Limited
Subject Keyword	Immunology and Microbiology (miscellaneous) Biochemistry, Genetics and Molecular Biology(all) Medicine (miscellaneous) Neuroscience (miscellaneous) Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Neuroscience Immunology and Microbiology Medicine Biochemistry, Genetics and Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Sestrin-2, a repressor of PDGFRβ signalling, promotes cigarette-smoke-induced pulmonary emphysema in mice and is upregulated in individuals with COPD

Nanoparticulate carbon black in cigarette smoke induces DNA cleavage and Th17-mediated emphysema

Angiotensin-(1-7) attenuates disuse skeletal muscle atrophy in mice via its receptor, Mas

Altered RNA splicing contributes to skeletal muscle pathology in Kennedy disease knock-in mice

Nanoparticulate carbon black in cigarette smoke induces DNA cleavage and Th17-mediated emphysema

Long-lasting alterations to DNA methylation and ncRNAs could underlie the effects of fetal alcohol exposure in mice

Muscular dystrophy begins early in embryonic development deriving from stem cell loss and disrupted skeletal muscle formation

Muscle dysfunction in mice with smoke-induced COPD

Concurrent dual allergen exposure and its effects on airway hyperresponsiveness, inflammation and remodeling in mice

Long-term nose-only cigarette smoke exposure induces emphysema and mild skeletal muscle dysfunction in mice

Similar Documents

Sestrin-2, a repressor of PDGFRβ signalling, promotes cigarette-smoke-induced pulmonary emphysema in mice and is upregulated in individuals with COPD

Nanoparticulate carbon black in cigarette smoke induces DNA cleavage and Th17-mediated emphysema

Angiotensin-(1-7) attenuates disuse skeletal muscle atrophy in mice via its receptor, Mas

Altered RNA splicing contributes to skeletal muscle pathology in Kennedy disease knock-in mice

Nanoparticulate carbon black in cigarette smoke induces DNA cleavage and Th17-mediated emphysema

Long-lasting alterations to DNA methylation and ncRNAs could underlie the effects of fetal alcohol exposure in mice

Muscular dystrophy begins early in embryonic development deriving from stem cell loss and disrupted skeletal muscle formation

Muscle dysfunction in mice with smoke-induced COPD

Concurrent dual allergen exposure and its effects on airway hyperresponsiveness, inflammation and remodeling in mice

Long-term nose-only cigarette smoke exposure induces emphysema and mild skeletal muscle dysfunction in mice