NDLI: Sm protein down-regulation leads to defects in nuclear pore complex disassembly and distribution in C. elegans embryos

Content Provider	PubMed Central
Author	Joseph-strauss, Daphna Gorjánácz, Mátyás Rachel, Santarella-mellwig Voronina, Ekaterina Audhya, Anjon Orna, Cohen-fix
Abstract	Nuclear pore complexes (NPCs) are large macromolecular structures embedded in the nuclear envelope (NE), where they facilitate exchange of molecules between the cytoplasm and the nucleoplasm. In most cell types, NPCs are evenly distributed around the NE. However, the mechanisms dictating NPC distribution are largely unknown. Here, we used the model organism C. elegans to identify genes that affect NPC distribution during early embryonic divisions. We found that down-regulation of the Sm proteins, which are core components of the spliceosome, but not down-regulation of other splicing factors, led to clustering of NPCs. Down-regulation of Sm proteins also led to incomplete disassembly of NPCs during mitosis, but had no effect on lamina disassembly, suggesting that the defect in NPC disassembly was not due to a general defect in nuclear envelope breakdown. We further found that these mitotic NPC remnants persisted on an ER membrane that juxtaposes the mitotic spindle. At the end of mitosis, the remnant NPCs moved toward the chromatin and the reforming NE, where they ultimately clustered by forming membrane stacks perforated by NPCs. Our results suggest a novel, splicing-independent, role for Sm proteins in NPC disassembly, and point to a possible link between NPC disassembly in mitosis and NPC distribution in the subsequent interphase.
Related Links	http://dx.doi.org/10.1016/j.ydbio.2012.02.036
Ending Page	457
Page Count	13
Starting Page	445
File Format	PDF
ISSN	00121606
e-ISSN	1095564X
Journal	Developmental Biology
Issue Number	2
Volume Number	365
Language	English
Publisher Date	2012-05-01
Access Restriction	Open
Subject Keyword	Developmental Biology Cell Biology Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Molecular Biology

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