NDLI: Probiotics stimulate enterocyte migration and microbial diversity in the neonatal mouse intestine

Content Provider	PubMed Central
Author	Preidis, Geoffrey A. Saulnier, Delphine M. Blutt, Sarah E. Mistretta, Toni-ann Riehle, Kevin P. Major, Angela M. Venable, Susan F. Finegold, Milton J. Petrosino, Joseph F. Conner, Margaret E. James, Versalovic
Copyright Year	2012
Abstract	Beneficial microbes and probiotics show promise for the treatment of pediatric gastrointestinal diseases. However, basic mechanisms of probiosis are not well understood, and most investigations have been performed in germ-free or microbiome-depleted animals. We sought to functionally characterize probiotic-host interactions in the context of normal early development. Outbred CD1 neonatal mice were orally gavaged with one of two strains of human-derived Lactobacillus reuteri or an equal volume of vehicle. Transcriptome analysis was performed on enterocyte RNA isolated by laser-capture microdissection. Enterocyte migration and proliferation were assessed by labeling cells with 5-bromo-2′-deoxyuridine, and fecal microbial community composition was determined by 16S metagenomic sequencing. Probiotic ingestion altered gene expression in multiple canonical pathways involving cell motility. L. reuteri strain DSM 17938 dramatically increased enterocyte migration (3-fold), proliferation (34%), and crypt height (29%) compared to vehicle-treated mice, whereas strain ATCC PTA 6475 increased cell migration (2-fold) without affecting crypt proliferative activity. In addition, both probiotic strains increased the phylogenetic diversity and evenness between taxa of the fecal microbiome 24 h after a single probiotic gavage. These experiments identify two targets of probiosis in early development, the intestinal epithelium and the gut microbiome, and suggest novel mechanisms for probiotic strain-specific effects.—Preidis, G. A., Saulnier, D. M., Blutt, S. E., Mistretta,T.-A., Riehle, K. P., Major, A. M., Venable, S. F., Finegold, M. J., Petrosino, J. F., Conner, M. E., Versalovic, J. Probiotics stimulate enterocyte migration and microbial diversity in the neonatal mouse intestine.
Related Links	http://dx.doi.org/10.1096/fj.10-177980
Ending Page	1969
Page Count	10
Starting Page	1960
File Format	PDF
ISSN	08926638
e-ISSN	15306860
Journal	The FASEB Journal
Issue Number	5
Volume Number	26
Language	English
Publisher	Federation of American Societies for Experimental Biology
Publisher Date	2012-05-01
Access Restriction	Open
Rights Holder	Federation of American Societies for Experimental Biology
Subject Keyword	Biotechnology Genetics Biochemistry Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Molecular Biology Biochemistry Biotechnology

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