NDLI: Inhibition of the p66/p51 form of human immunodeficiency virus reverse transcriptase by tRNA(Lys).

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Organization of the mitochondrial genome of Atlantic cod, Gadus morhua.

Stereoalignment requirements for activation of transcription by the simian virus 40 enhancer.

Inhibition of the p66/p51 form of human immunodeficiency virus reverse transcriptase by tRNA(Lys).

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Sequence of cbh-1 gene of Humicola grisea var. thermoidea.

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Nucleotide sequence of the alternatively spliced human thyroid peroxidase cDNA, TPO-2.

DNA sequence of a beta-glucosidase from Ruminococcus albus.

Sequence of E.coli K12 valU operon which contains 3 genes for tRNA(Val1) and 1 gene for tRNA(Lys).

Nucleotide sequence of mouse virus-like (VL30) retrotransposon BVL-1.

The complete sequence of mag, a new retrotransposon in Bombyx mori.

Nucleotide sequence of recA gene of Aquaspirillum magnetotacticum.

Nucleotide sequence of the bovine insulin-like growth factor 1 (IGF-1) and its IGF-1A precursor.

Sequence of the cDNA encoding ITF-1, a positive-acting transcription factor.

Sequence of the cDNA encoding ITF-2, a positive-acting transcription factor.

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Human cDNA sequence homologous to the mouse LLRep3 gene family.

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Nucleotide sequence of a cloned rice genomic DNA fragment that encodes a 10 kDa prolamin polypeptide.

Sequence of a cDNA specifying subunit VIIc of human cytochrome c oxidase.

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An additional HindIII polymorphism at the coagulation factor XIIIA locus

StuI RFLP in the human ABL locus

New nucleotide sequence data on the EMBL File Server

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Inhibition of the p66/p51 form of human immunodeficiency virus reverse transcriptase by tRNA(Lys).

Content Provider	PubMed Central
Author	Bordier, B. Tarrago-litvak, L. Sallafranque-andreola, M. L. Robert, D. Tharaud, D. Fournier, M. Barr, P. J. Litvak, S. Sarih-cottin, L.
Abstract	Human immunodeficiency virus (HIV) reverse transcriptase (RT) uses host tRNA(Lys) partially annealed to the primer binding site (PBS) as primer for the initiation of cDNA synthesis. When assaying cDNA synthesis with a template-primer complex formed by an RNA fragment carrying the PBS site and bovine tRNA(Lys) we noticed that an excess of primer tRNA inhibited strongly the DNA polymerase activity of a recombinant HIV RT (p66-p51 heterodimeric form) produced in transformed yeast cells. The same inhibitory effect was observed with animal DNA polymerase alpha, while avian retrovirus RT was neither affected by tRNA(Lys) nor by its specific primer tRNA(Trp). Although the strongest inhibition was observed with tRNA(Lys), other tRNas like tRNA(Phe) and tRNA(Trp) inhibited also the HIV RT, whereas tRNAs specific for valine, proline and glycine had no effect on enzyme activity. Digestion of tRNA(Lys) with pancreatic RNase abolished the inhibition; on the other hand T1 RNase digestion had no effect on the inhibition suggesting a role of the anticodon region in this effect. The 12- and 14-mers corresponding to the anticodon regions of the three bovine tRNA(Lys) isoacceptors inhibited RT activity, indicating that at least an important part of the inhibitory effect could be ascribed to this tRNA region. A strong stimulation of DNA polymerase activity was observed when the effect of tRNA(Lys) was assayed on a recombinant HIV reverse transcriptase produced in a protease deficient yeast strain, which leads to the production of an active p66 enzyme. The same tRNAs that inhibited strongly the heterodimeric form stimulated the p66 form of HIV reverse transcriptase. The results suggest that although both enzymatic forms are able to interact with tRNA(Lys) the topography, as well as the functional implications of the interaction between the precursor and the mature form of HIV reverse transcriptase with the tRNA(Lys) primer, are different.
Starting Page	429
File Format	PDF
ISSN	13624962
e-ISSN	13624962
Journal	Nucleic Acids Research
Issue Number	3
Volume Number	18
Language	English
Publisher Date	1990-02-11
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics

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