NDLI: Ins(1,4,5) P 3 receptor-mediated Ca2+ signaling and autophagy induction are interrelated

Content Provider	PubMed Central
Author	Jean-paul, Decuypere Kirsten, Welkenhuyzen Luyten, Tomas Ponsaerts, Raf Dewaele, Michael Jordi, Molgó Patrizia, Agostinis Missiaen, Ludwig De Smedt, Humbert Parys, Jan B. Bultynck, Geert
Copyright Year	2011
Abstract	The role of intracellular Ca2+ signaling in starvation-induced autophagy remains unclear. Here, we examined Ca2+ dynamics during starvation-induced autophagy and the underlying molecular mechanisms. Tightly correlating with autophagy stimulation, we observed a remodeling of the Ca2+ signalosome. First, short periods of starvation (1 to 3 h) caused a prominent increase of the ER Ca2+-store content and enhanced agonist-induced Ca2+ release. The mechanism involved the upregulation of intralumenal ER Ca2+-binding proteins, calreticulin and Grp78/BiP, which increased the ER Ca2+-buffering capacity and reduced the ER Ca2+ leak. Second, starvation led to Ins(1,4,5)P 3R sensitization. Immunoprecipitation experiments showed that during starvation Beclin 1, released from Bcl-2, first bound with increasing efficiency to Ins(1,4,5)P 3Rs; after reaching a maximal binding after 3 h, binding, however, decreased again. The interaction site of Beclin 1 was determined to be present in the N-terminal Ins(1,4,5)P 3-binding domain of the Ins(1,4,5)P 3R. The starvation-induced Ins(1,4,5)P 3R sensitization was abolished in cells treated with BECN1 siRNA, but not with ATG5 siRNA, pointing toward an essential role of Beclin 1 in this process. Moreover, recombinant Beclin 1 sensitized Ins(1,4,5)P 3Rs in 45Ca2+-flux assays, indicating a direct regulation of Ins(1,4,5)P 3R activity by Beclin 1. Finally, we found that Ins(1,4,5)P 3R-mediated Ca2+ signaling was critical for starvation-induced autophagy stimulation, since the Ca2+ chelator BAPTA-AM as well as the Ins(1,4,5)P 3R inhibitor xestospongin B abolished the increase in LC3 lipidation and GFP-LC3-puncta formation. Hence, our results indicate a tight and essential interrelation between intracellular Ca2+ signaling and autophagy stimulation as a proximal event in response to starvation.
Related Links	http://dx.doi.org/10.4161/auto.7.12.17909
Ending Page	1489
Page Count	18
Starting Page	1472
File Format	PDF
ISSN	15548627
e-ISSN	15548635
Journal	Autophagy
Issue Number	12
Volume Number	7
Language	English
Publisher	Landes Bioscience
Publisher Date	2011-12-01
Access Restriction	Open
Rights Holder	Landes Bioscience
Subject Keyword	Cell Biology Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Biology

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