NDLI: Identification of the cglC, cglD, cglE, and cglF Genes and Their Role in Cell Contact-Dependent Gliding Motility in Myxococcus xanthus

Content Provider	PubMed Central
Author	Pathak, Darshankumar T. Wall, Daniel
Copyright Year	2012
Abstract	Within Myxococcus xanthus biofilms, cells actively move and exchange their outer membrane (OM) lipoproteins and lipids. Between genetically distinct strains, OM exchange can regulate recipient cell behaviors, including gliding motility and development. Although many different proteins are thought to be exchanged, to date, only two endogenous OM lipoproteins, CglB and Tgl, are known to be transferred. Protein exchange requires the TraAB proteins in recipient and donor cells, where they are hypothesized to facilitate OM fusion for transfer. To better understand the types of proteins exchanged, we identified the genes for the remaining set of cgl gliding motility mutants. These mutants are unique because their motility defect can be transiently restored by physical contact with donor cells that encode the corresponding wild-type protein, a process called stimulation. Similar to CglB and Tgl, the cglC and cglD genes encode type II signal sequences, suggesting that they are also lipoproteins. Surprisingly, the cglE and cglF genes instead encode type I signal sequences, suggesting that nonlipoproteins are also exchanged. Consistent with this idea, the addition of exogenous synthetic CglF protein (71 amino acids) to a cglF mutant rescued its motility defect. In contrast to a live donor cell, stimulation with purified CglF protein occurred independently of TraA. These results also indicate that CglF may localize to the cell surface. The implications of our findings on OM exchange are discussed.
Related Links	http://dx.doi.org/10.1128/JB.00055-12
Starting Page	1940
File Format	PDF
ISSN	10985530
e-ISSN	10985530
Journal	Journal of Bacteriology
Issue Number	8
Volume Number	194
Language	English
Publisher	American Society for Microbiology
Publisher Date	2012-04-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Microbiology

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