NDLI: DNaseI hypersensitive sites 1, 2 and 3 of the human beta-globin dominant control region direct position-independent expression.

Please wait, while we are loading the content...

Year: 2016, Volume: 44

Year: 2015, Volume: 43

Year: 2014, Volume: 42

Year: 2013, Volume: 41

Year: 2012, Volume: 40

Year: 2011, Volume: 39

Year: 2010, Volume: 38

Year: 2009, Volume: 37

Year: 2008, Volume: 37

Year: 2008, Volume: 36

Year: 2007, Volume: 36

Year: 2007, Volume: 35

Year: 2006, Volume: 35

Year: 2006, Volume: 34

Year: 2005, Volume: 33

Year: 2004, Volume: 33

Year: 2004, Volume: 32

Year: 2003, Volume: 31

Year: 2002, Volume: 30

Year: 2001, Volume: 29

Year: 2000, Volume: 28

Year: 1999, Volume: 27

Year: 1998, Volume: 26

Year: 1997, Volume: 25

Year: 1996, Volume: 24

Year: 1995, Volume: 23

Year: 1994, Volume: 22

Year: 1993, Volume: 21

Year: 1992, Volume: 20

Year: 1991, Volume: 19

Year: 1990, Volume: 18

Year: 1990, Volume: 18, Issue: Suppl

Year: 1990, Volume: 18, Issue: 24

Year: 1990, Volume: 18, Issue: 23

Year: 1990, Volume: 18, Issue: 22

Year: 1990, Volume: 18, Issue: 21

Year: 1990, Volume: 18, Issue: 20

Year: 1990, Volume: 18, Issue: 19

Year: 1990, Volume: 18, Issue: 18

Year: 1990, Volume: 18, Issue: 17

Year: 1990, Volume: 18, Issue: 16

Year: 1990, Volume: 18, Issue: 15

Year: 1990, Volume: 18, Issue: 14

Year: 1990, Volume: 18, Issue: 13

Year: 1990, Volume: 18, Issue: 12

Efficient site directed in vitro mutagenesis using ampicillin selection.

A temperature-sensitive mutant of Escherichia coli affected in the alpha subunit of RNA polymerase.

U6 snRNA genes of Arabidopsis are transcribed by RNA polymerase III but contain the same two upstream promoter elements as RNA polymerase II-transcribed U-snRNA genes.

Amplification of plant U3 and U6 snRNA gene sequences using primers specific for an upstream promoter element and conserved intragenic regions.

Isolation and characterization of two novel, closely related ATF cDNA clones from HeLa cells.

A human immunoglobulin kappa orphon without sequence defects may be the product of a pericentric inversion.

Interaction of RNase P from Escherichia coli with pseudoknotted structures in viral RNAs.

Three genes under different developmental control encode elongation factor 1-alpha in Xenopus laevis.

Poly(dA).poly(dT) rich sequences are not sufficient to exclude nucleosome formation in a constitutive yeast promoter.

DNaseI hypersensitive sites 1, 2 and 3 of the human beta-globin dominant control region direct position-independent expression.

A mammalian temperature-sensitive mutation affecting G1 progression results from a single amino acid substitution in asparagine synthetase.

Cloning, in vitro transcription, and biological activity of Escherichia coli 23S ribosomal RNA.

Use of synthetic oligoribonucleotides to probe RNA-protein interactions in the MS2 translational operator complex.

Transcriptional slippage occurs during elongation at runs of adenine or thymine in Escherichia coli.

Effects of oligo sequence and chemistry on the efficiency of oligodeoxyribonucleotide-mediated mRNA cleavage.

Sequence dependent effects in methylphosphonate deoxyribonucleotide double and triple helical complexes.

Spectroscopic and calorimetric investigation on the DNA triplex formed by d(CTCTTCTTTCTTTTCTTTCTTCTC) and d(GAGAAGAAAGA) at acidic pH.

Lymphoid-specific transcriptional activation by components of the IgH enhancer: studies on the E2/E3 and octanucleotide elements.

Herpes simplex virus helicase-primase: the UL8 protein is not required for DNA-dependent ATPase and DNA helicase activities.

Recombination between directly repeated origins of conjugative transfer cloned in M13 bacteriophage DNA models ligation of the transferred plasmid strand.

Advanced mammalian gene transfer: high titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line.

E. coli promoter spacer regions contain nonrandom sequences which correlate to spacer length.

Unusual c-fos induction upon chromaffin PC12 differentiation by sodium butyrate: loss of fos autoregulatory function.

Transcription factor IIA of wheat and human function similarly with plant and animal viral promoters.

Transcriptional and post-transcriptional processes regulate expression of RNA encoding the small subunit of ribulose-1,5-biphosphate carboxylase differently in petunia and in soybean.

Nucleotide sequence of the HPV16 L1 open reading frame.

Nucleotide sequence of a pseudogene for pea phytochrome reminiscent of an incorrect splicing event.

Nucleotide sequence of the HA gene of influenza B/Beijing/1/87.

Nucleotide and amino acid polymorphism in the gene for L-histidinol dehydrogenase of Escherichia coli K12.

Cloning and sequencing of bacteriophage T4 genes between map positions 128.3-130.3.

Nucleotide and deduced amino acid sequence for the mouse homologue of the rat T-cell differentiation marker RT6.

Sequence coding for a sexual stage specific protein of Plasmodium falciparum.

Nucleotide sequences of cDNAs alpha and gamma enolase mRNAs from mouse brain.

The nucleotide sequence of the mouse cDNA encoding the beta subunit of casein kinase II.

Sequence of D alpha 2, a novel alpha-like subunit of Drosophila nicotinic acetylcholine receptors.

Complete sequence of the rabbit whey acidic protein gene.

Nucleotide sequence of a rat liver cDNA encoding the cytosolic 3-hydroxy-3-methylglutaryl coenzyme A synthase.

Nucleotide sequence of cottonseed malate synthase.

Nucleotide sequence of the psaC gene of the cyanobacterium Synechococcus vulcanus.

Nucleotide sequence of a cDNA for mouse cytochrome c oxidase subunit VIIc.

Boll weevil testis-specific cDNA.

Complete sequence and organization of the human cardiac beta-myosin heavy chain gene.

Sequence of human liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.

Nucleotide sequence of the yeast SDH1 gene encoding a serine dehydratase homolog.

Nucleotide sequence of an actin cDNA gene from Aplysia californica.

Cloning and sequencing of a zinc finger cDNA expressed in mouse testis.

Sequence analysis of the iclR gene encoding the repressor of the acetate operon in Salmonella typhimurium.

Nucleotide sequence of the gene encoding the duck embryonic epsilon-globin.

Cloning and sequencing of a bovine preproendothelin cDNA.

Nucleotide sequence of the Thermus thermophilus HB8 rps12 and rps7 genes coding for the ribosomal proteins S12 and S7.

Nucleotide sequence of the erythromycin resistance gene of the conjugative transposon Tn1545.

Nucleotide sequence of a maize U6 gene.

The complete sequence of the capsid p40 gene from infectious laryngotracheitis virus.

Digestion conditions resulting in altered cut site specificity for HinfI.

Altered specificity of restriction endonuclease HinfI.

The restriction enzyme EheI (GGC/GCC) is sensitive to CpG methylation.

pSH4: a mammalian cDNA expression vector.

Purification of mRNA directly from crude plant tissues in 15 minutes using magnetic oligo dT microspheres.

GST1 gene deletion determined by polymerase chain reaction.

Primer extension technique for the detection of single nucleotide in genomic DNA.

PCR detection of two RFLP's at the D21S13 locus

RFLP for BglII at the human tyrosinase (TYR) locus

A BstE II RFLP at the DXS153 locus

A RsaI RFLP at the DXS467 locus

New nucleotide sequence data on the EMBL File Server

Structure and transcription of a human gene for H1 RNA, the RNA component of human RNase P

Year: 1990, Volume: 18, Issue: 11

Year: 1990, Volume: 18, Issue: 10

Year: 1990, Volume: 18, Issue: 9

Year: 1990, Volume: 18, Issue: 8

Year: 1990, Volume: 18, Issue: 7

Year: 1990, Volume: 18, Issue: 6

Year: 1990, Volume: 18, Issue: 5

Year: 1990, Volume: 18, Issue: 4

Year: 1990, Volume: 18, Issue: 3

Year: 1990, Volume: 18, Issue: 2

Year: 1990, Volume: 18, Issue: 1

Year: 1989, Volume: 17

Year: 1988, Volume: 16

Year: 1987, Volume: 15

Year: 1986, Volume: 14

Year: 1985, Volume: 13

Year: 1984, Volume: 12

Year: 1983, Volume: 11

Year: 1982, Volume: 10

Year: 1981, Volume: 9

Year: 1980, Volume: 8

Year: 1979, Volume: 7

Year: 1979, Volume: 6

Year: 1978, Volume: 5

Year: 1977, Volume: 4

Year: 1976, Volume: 3

Year: 1975, Volume: 2

Year: 1974, Volume: 1

DNaseI hypersensitive sites 1, 2 and 3 of the human beta-globin dominant control region direct position-independent expression.

Content Provider	PubMed Central
Author	Fraser, P. Hurst, J. Collis, P. Grosveld, F.
Abstract	The human beta-globin dominant control region (DCR) which flanks the multigene beta-globin locus directs high level, site of integration independent, copy number dependent expression on a linked human beta-globin gene in transgenic mice and stably transfected mouse erythroleukemia (MEL) cells. We have assayed each of the individual DNaseI hypersensitive regions present in the full 15kb DCR for position independence and copy number dependence of a linked beta-globin gene in transgenic mice. The results show that at least three of the individual DNaseI hypersensitive site regions (sites 1, 2 and 3), though expressing at lower levels than the full DCR, are capable of position independent, copy number dependent expression. Site 2 alone directs the highest level of expression of the single site constructs, producing nearly 70% of the level of the full DCR. Sites 1 and 3 each provide 30% of the full activity. Deletion of either site 2 or 3 from the complete set significantly reduces the level of expression, but does not effect position independence or copy number dependence. This demonstrates that sites 2 and 3 are required for full expression and suggests that all the sites are required for the full expression of even a single gene from this multigene locus.
Starting Page	3503
File Format	PDF
ISSN	13624962
e-ISSN	13624962
Journal	Nucleic Acids Research
Issue Number	12
Volume Number	18
Language	English
Publisher Date	1990-06-25
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in