NDLI: Fucose-Containing Sulfated Polysaccharides from Brown Seaweeds Inhibit Proliferation of Melanoma Cells and Induce Apoptosis by Activation of Caspase-3 in Vitro

Content Provider	PubMed Central
Author	Ale, Marcel Tutor Maruyama, Hiroko Tamauchi, Hidekazu Mikkelsen, Jørn D. Meyer, Anne S.
Copyright Year	2011
Abstract	Fucose-containing sulfated polysaccharides (FCSPs) extracted from seaweeds, especially brown macro-algae, are known to possess essential bioactive properties, notably growth inhibitory effects on tumor cells. In this work, we conducted a series of in vitro studies to examine the influence of FCSPs products from Sargassum henslowianum C. Agardh (FSAR) and Fucus vesiculosus (FVES), respectively, on proliferation of melanoma B16 cells and to investigate the underlying apoptosis promoting mechanisms. Cell viability analysis showed that both FCSPs products, i.e., FSAR and FVES, decreased the proliferation of the melanoma cells in a dose-response fashion, with FSAR being more potent at lower dosages, and FVES being relatively more anti-proliferative than FSAR at higher dosages. Flow cytometric analysis by Annexin V staining of the melanoma cells exposed to the FCSPs products confirmed that both FSAR and FVES induced apoptosis. The FCSPs-induced apoptosis was evidenced by loss of plasma membrane asymmetry and translocation of the cell membrane phospholipids and was accompanied by the activation of caspase-3. The FCSPs bioactivity is proposed to be attributable to distinct structural features of the FCSPs, particularly the presence of sulfated galactofucans (notably in S. henslowianum) and sulfated fucans (notably in F. vesiculosus). This study thus indicates that unfractionated FCSPs may exert bioactive effects on skin cancer cells via induction of apoptosis through cascades of reactions that involve activation of caspase-3.
Related Links	http://dx.doi.org/10.3390/md9122605
Ending Page	2621
Page Count	17
Starting Page	2605
File Format	PDF
ISSN	16603397
e-ISSN	16603397
Journal	Marine Drugs
Issue Number	12
Volume Number	9
Language	English
Publisher	MDPI
Publisher Date	2011-12-01
Access Restriction	Open
Rights Holder	MDPI
Subject Keyword	Drug Discovery Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Drug Discovery Pharmaceutical Science

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