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Identification of Isocitrate Dehydrogenase 1 as a Potential Diagnostic and Prognostic Biomarker for Non-small Cell Lung Cancer by Proteomic Analysis*
| Content Provider | PubMed Central |
|---|---|
| Author | Tan, Fengwei Jiang, Ying Sun, Nan Chen, Zhaoli Lv, Yongzhuang Shao, Kang Li, Ning Qiu, Bin Gao, Yibo Li, Baozhong Tan, Xiaogang Zhou, Fang Wang, Zhen Ding, Dapeng Wang, Jiwen Sun, Jian Hang, Jie Shi, Susheng Feng, Xiaoli He, Fuchu He, Jie |
| Copyright Year | 2012 |
| Abstract | Lung cancer is the leading cause of cancer-related death in the world. To explore tumor biomarkers for clinical application, two-dimensional fluorescence difference gel electrophoresis and subsequent MALDI-TOF/TOF mass spectrometry were performed to identify proteins differentially expressed in 12 pairs of lung squamous cell tumors and their corresponding normal tissues. A total of 28 nonredundant proteins were identified with significant alteration in lung tumors. The up-regulation of isocitrate dehydrogenase 1 (IDH1), superoxide dismutase 2, 14-3-3ε, and receptor of activated protein kinase C1 and the down-regulation of peroxiredoxin 2 in tumors were validated by RT-PCR and Western blot analysis in independent 15 pairs of samples. Increased IDH1 expression was further verified by the immunohistochemical study in extended 73 squamous cell carcinoma and 64 adenocarcinoma clinical samples. A correlation between IDH1 expression and poor overall survival of non-small cell lung cancer (NSCLC) patients was observed. Furthermore, ELISA analysis showed that the plasma level of IDH1 was significantly elevated in NSCLC patients compared with benign lung disease patients and healthy individuals. In addition, knockdown of IDH1 by RNA interference suppressed the proliferation of NSCLC cell line and decreased the growth of xenograft tumors in vivo. These observations suggested that IDH1, as a protein promoting tumor growth, could be used as a plasma biomarker for diagnosis and a histochemical biomarker for prognosis prediction of NSCLC. |
| Related Links | http://dx.doi.org/10.1074/mcp.m111.008821 |
| File Format | |
| ISSN | 15359476 |
| e-ISSN | 15359484 |
| Journal | Molecular & Cellular Proteomics : MCP |
| Issue Number | 2 |
| Volume Number | 11 |
| Language | English |
| Publisher | The American Society for Biochemistry and Molecular Biology |
| Publisher Date | 2012-02-01 |
| Access Restriction | Open |
| Rights Holder | The American Society for Biochemistry and Molecular Biology |
| Subject Keyword | Analytical Chemistry Biochemistry Molecular Biology Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Analytical Chemistry Molecular Biology Biochemistry |
