NDLI: Brush border Myosin Ia has tumor suppressor activity in the intestine

Content Provider	PubMed Central
Author	Mazzolini, Rocco Dopeso, Higinio Silvia, Mateo-lozano Chang, Wakam Rodrigues, Paulo Sarah, Bazzocco Hafid, Alazzouzi Landolfi, Stefania Javier, Hernández-losa Andretta, Elena Pia, Alhopuro Espín, Eloy Armengol, Manel Tabernero, Josep Cajal, Santiago Ramón Y. Kloor, Matthias Gebert, Johannes Mariadason, John M. Schwartz, Simo Aaltonen, Lauri A. Mooseker, Mark S. Arango, Diego
Abstract	The loss of the epithelial architecture and cell polarity/differentiation is known to be important during the tumorigenic process. Here we demonstrate that the brush border protein Myosin Ia (MYO1A) is important for polarization and differentiation of colon cancer cells and is frequently inactivated in colorectal tumors by genetic and epigenetic mechanisms. MYO1A frame-shift mutations were observed in 32% (37 of 116) of the colorectal tumors with microsatellite instability analyzed, and evidence of promoter methylation was observed in a significant proportion of colon cancer cell lines and primary colorectal tumors. The loss of polarization/differentiation resulting from MYO1A inactivation is associated with higher tumor growth in soft agar and in a xenograft model. In addition, the progression of genetically and carcinogen-initiated intestinal tumors was significantly accelerated in Myo1a knockout mice compared with Myo1a wild-type animals. Moreover, MYO1A tumor expression was found to be an independent prognostic factor for colorectal cancer patients. Patients with low MYO1A tumor protein levels had significantly shorter disease-free and overall survival compared with patients with high tumoral MYO1A (logrank test P = 0.004 and P = 0.009, respectively). The median time-to-disease recurrence in patients with low MYO1A was 1 y, compared with >9 y in the group of patients with high MYO1A. These results identify MYO1A as a unique tumor-suppressor gene in colorectal cancer and demonstrate that the loss of structural brush border proteins involved in cell polarity are important for tumor development.
Related Links	http://dx.doi.org/10.1073/pnas.1108411109
Ending Page	1535
Page Count	6
Starting Page	1530
File Format	PDF
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	5
Volume Number	109
Language	English
Publisher	National Academy of Sciences
Publisher Date	2012-01-31
Access Restriction	Open
Rights Holder	National Academy of Sciences
Subject Keyword	General Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Brush border myosin Ia has tumor suppressor activity in the intestine.

Myosin Ia is required for CFTR brush border membrane trafficking and ion transport in the mouse small intestine

Kinetic characterization of brush border myosin-I ATPase

Actin in the Brush-Border of Epithelial Cells of the Chicken Intestine

MYO1A (brush border myosin I) dynamics in the brush border of LLC-PK1-CL4 cells.

Calmodulin dissociation regulates brush border myosin I (110-kD- calmodulin) mechanochemical activity in vitro

Parallel modulation of brush border myosin conformation and enzyme activity induced by monoclonal antibodies

Particulate' hexokinase activity in rat intestine. The comparative contributions of mitochondria and brush-border membranes.

Actin in the brush-border of epithelial cells of the chicken intestine.

Brush border Myosin Ia has tumor suppressor activity in the intestine

Similar Documents

Brush border myosin Ia has tumor suppressor activity in the intestine.

Myosin Ia is required for CFTR brush border membrane trafficking and ion transport in the mouse small intestine

Kinetic characterization of brush border myosin-I ATPase

Actin in the Brush-Border of Epithelial Cells of the Chicken Intestine

MYO1A (brush border myosin I) dynamics in the brush border of LLC-PK1-CL4 cells.

Calmodulin dissociation regulates brush border myosin I (110-kD- calmodulin) mechanochemical activity in vitro

Parallel modulation of brush border myosin conformation and enzyme activity induced by monoclonal antibodies

Particulate' hexokinase activity in rat intestine. The comparative contributions of mitochondria and brush-border membranes.

Actin in the brush-border of epithelial cells of the chicken intestine.

Brush border Myosin Ia has tumor suppressor activity in the intestine