NDLI: Real-Time PCR Analysis of the Intestinal Microbiotas in Peritoneal Dialysis Patients

Content Provider	PubMed Central
Author	Wang, I-kuan Lai, Hsueh-chou Yu, Cheng-ju Liang, Chih-chia Chang, Chiz-tzung Kuo, Huey-liang Yang, Ya-fei Lin, Chung-chih Lin, Hsin-hung Liu, Yao-lung Chang, Yi-chih Wu, Yi-ying Chen, Chu-huang Li, Chi-yuan Chuang, Feng-rong Huang, Chiu-ching Lin, Chih-hsueh Lin, Hung-chih
Copyright Year	2012
Abstract	Bifidobacterium and Lactobacillus can beneficially affect the host by producing acetic acid and lactic acid, which lower pH and thereby inhibit the growth of pathogens or allow the probiotic bacteria to compete with pathogens for epithelial adhesion sites and nutrients. The transmural migration of enteric organisms into the peritoneal cavity can cause peritonitis in peritoneal dialysis (PD) patients. We hypothesized that the composition of the intestinal microbiota with regard to Lactobacillus species and Bifidobacterium species differed between PD patients and healthy controls. The aim of the study was to investigate these differences by real-time PCR analysis of fecal samples. From 1 August 2009 to 31 March 2010, a total of 29 nondiabetic PD patients and 41 healthy controls from China Medical University Hospital were recruited after giving their informed consent. Fecal samples were collected from the PD patients and their age-matched counterparts in the morning using a standardized procedure. DNA extracted from these samples was analyzed by real-time PCR. All bifidobacteria, Bifidobacterium catenulatum, B. longum, B. bifidum, Lactobacillus plantarum, L. paracasei, and Klebsiella pneumoniae were less frequently detected in the patient samples. Dysbiosis (microbial imbalance) may impair intestinal barrier function and increase host vulnerability to pathogen invasion. Further studies are necessary to confirm our findings before clinical trials with probiotic supplementation in PD patients.
Related Links	http://dx.doi.org/10.1128/aem.05605-11
Ending Page	1112
Page Count	6
Starting Page	1107
File Format	PDF
ISSN	00992240
e-ISSN	10985336
Journal	Applied and Environmental Microbiology
Issue Number	4
Volume Number	78
Language	English
Publisher	American Society for Microbiology
Publisher Date	2012-02-15
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Biotechnology Food Science Ecology Applied Microbiology and Biotechnology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Ecology Food Science Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Real-Time PCR Analysis of Vibrio vulnificus from Oysters

Comparison between Cultured Small-Intestinal and Fecal Microbiotas in Beagle Dogs

Use of Real-Time Quantitative PCR for the Analysis of φLC3 Prophage Stability in Lactococci

Diagnostic Real-Time PCR for Detection of Salmonella in Food

Power Analysis for Real-Time PCR Quantification of Genes in Activated Sludge and Analysis of the Variability Introduced by DNA Extraction

Comparison of Real-Time PCR, Reverse Transcriptase Real-Time PCR, Loop-Mediated Isothermal Amplification, and the FDA Conventional Microbiological Method for the Detection of Salmonella spp. in Produce ▿ †

Quantitative Real-Time Reverse Transcription-PCR Analysis of Deformed Wing Virus Infection in the Honeybee (Apis mellifera L.)

Real-Time PCR Method for Detection of Pathogenic Yersinia enterocolitica in Food▿

Real-Time PCR Quantitation of Clostridia in Feces of Autistic Children

Real-Time PCR Analysis of the Intestinal Microbiotas in Peritoneal Dialysis Patients

Similar Documents

Real-Time PCR Analysis of Vibrio vulnificus from Oysters

Comparison between Cultured Small-Intestinal and Fecal Microbiotas in Beagle Dogs

Use of Real-Time Quantitative PCR for the Analysis of φLC3 Prophage Stability in Lactococci

Diagnostic Real-Time PCR for Detection of Salmonella in Food

Power Analysis for Real-Time PCR Quantification of Genes in Activated Sludge and Analysis of the Variability Introduced by DNA Extraction

Comparison of Real-Time PCR, Reverse Transcriptase Real-Time PCR, Loop-Mediated Isothermal Amplification, and the FDA Conventional Microbiological Method for the Detection of Salmonella spp. in Produce ▿ †

Quantitative Real-Time Reverse Transcription-PCR Analysis of Deformed Wing Virus Infection in the Honeybee (Apis mellifera L.)

Real-Time PCR Method for Detection of Pathogenic Yersinia enterocolitica in Food▿

Real-Time PCR Quantitation of Clostridia in Feces of Autistic Children

Real-Time PCR Analysis of the Intestinal Microbiotas in Peritoneal Dialysis Patients