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| Content Provider | PubMed Central |
|---|---|
| Author | He, Mei Novak, Jan Julian, Bruce A. Herr, Amy E. |
| Abstract | Aberrant glycosylation plays a pivotal role in a diverse set of diseases including cancer. A microfluidic lectin blotting platform is introduced to enable and expedite the identification of protein glycosylation based on protein size and affinity for specific lectins. The integrated multi-stage assay eliminates manual intervention steps required for slabgel lectin blotting, increases total assay throughput, limits reagent and sample consumption, and completes using one instrument. The assay is comprised of non-reducing sodiumdodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) followed by on-line post-sizing SDS filtration and lectin-based affinity blotting. Important functionality is conferred through both device and assay advances that enable integration of nanoporous membranes flanking a central microchamber to create sub-nanoliter volume compartments which trap SDS-protein complexes and allow electrophoretic SDS removal with buffer exchange. Recapitulation of protein binding for lectin was optimized through quantitative assessment of SDS-treated green fluorescent protein (GFP). Aberrantly glycosylated IgA1 with galactose-deficient O-glycans was probed in ~6 min from ~3 μL of sample. This new microfluidic lectin blotting provides a rapid and automated assay for assessment of aberrant glycosylation. |
| Related Links | http://dx.doi.org/10.1021/ja207963f |
| Starting Page | 19610 |
| File Format | |
| ISSN | 15205126 |
| e-ISSN | 15205126 |
| Journal | Journal of the American Chemical Society |
| Issue Number | 49 |
| Volume Number | 133 |
| Language | English |
| Publisher Date | 2011-12-14 |
| Access Restriction | Open |
| Subject Keyword | Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Chemistry Colloid and Surface Chemistry Biochemistry Catalysis |
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