NDLI: 3D structure of the Yersinia entomophaga toxin complex and implications for insecticidal activity

Content Provider	PubMed Central
Author	Landsberg, Michael J. Jones, Sandra A. Rosalba, Rothnagel Busby, Jason N. Marshall, Sean D. G. Simpson, Robert M. Lott, J. Shaun Hankamer, Ben Hurst, Mark R. H.
Abstract	Toxin complex (Tc) proteins are a class of bacterial protein toxins that form large, multisubunit complexes. Comprising TcA, B, and C components, they are of great interest because many exhibit potent insecticidal activity. Here we report the structure of a novel Tc, Yen-Tc, isolated from the bacterium Yersinia entomophaga MH96, which differs from the majority of bacterially derived Tcs in that it exhibits oral activity toward a broad range of insect pests, including the diamondback moth (Plutella xylostella). We have determined the structure of the Yen-Tc using single particle electron microscopy and studied its mechanism of toxicity by comparative analyses of two variants of the complex exhibiting different toxicity profiles. We show that the A subunits form the basis of a fivefold symmetric assembly that differs substantially in structure and subunit arrangement from its most well characterized homologue, the Xenorhabdus nematophila toxin XptA1. Histopathological and quantitative dose response analyses identify the B and C subunits, which map to a single, surface-accessible region of the structure, as the sole determinants of toxicity. Finally, we show that the assembled Yen-Tc has endochitinase activity and attribute this to putative chitinase subunits that decorate the surface of the TcA scaffold, an observation that may explain the oral toxicity associated with the complex.
Related Links	http://dx.doi.org/10.1073/pnas.1111155108
Ending Page	20549
Page Count	6
Starting Page	20544
File Format	PDF
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	51
Volume Number	108
Language	English
Publisher	National Academy of Sciences
Publisher Date	2011-12-20
Access Restriction	Open
Rights Holder	National Academy of Sciences
Subject Keyword	General Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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3D structure of the Yersinia entomophaga toxin complex and implications for insecticidal activity.

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